SUSCEPTIBILITY OF Salmonella typhi TO MEDICINAL PLANT EXTRACTS COMPARED TO CIPROFLOXACIN
Lukong Hubert Shalanyuy
, Samba Derick , Tanlaka Lucas Mengnjo , Samje Moses , Wam Elvis Chongsi
Key words: Susceptibility, Salmonella typhi, medicinal plant extracts, ciprofloxacin.
ABSTRACT The development of bacteria resistance to present available antibiotics has necessitated the needs to search for new effective and cheaper antibacterial agents. Resistance of Salmonella to antibiotics is a serious health problem in the world more especially in developing countries and Cameroon in particular. This study aimed at comparing the antibacterial activity of medicinal plant extracts (Cymbopogon citatus leaves, Carica papaya leaves, Bidens pilosa, and Mangifera indica leaves) used by traditional herbalist against Salmonella typhi, compared to Ciprofloxacin at the Standard Medical Diagnostic and Research Center – Bamenda. Plant samples were collected, dried away from sunlight and ground into powder form. 25g of each plant powder was weighed to form a pool mixture of medicinal plant powder and dispensed in 95% methanol and distilled water and kept still for 7days. Isolation of Salmonella typhi from human feces was done using Salmonella Shigella agar (SSA) and sub-cultured on Muella Hinton Agar. Two fold serial dilutions were made on the methanol and water while two methods of sensitivity testing (well diffusion and disc diffusion) were carried out on different Mueller Hinton agar plates and incubated at 37°C for 18-24hrs. The zones of inhibition were measured in mm, compared and analyzed in SPSS version 21. The sensitivity studies conducted on the medicinal plant extracts and ciprofloxacin indicated that the buffered alcohol extract inhibited S. typhi to a zone of 13.34mm and 7.45mm for distilled water extract (P˂0.05). Alcohol extract also recorded an inhibitory zone of 23.05mm by well diffusion and 19.55mm by disc diffusion (P˂0.05). Again, sensitivity studies using ciprofloxacin 5µg gave a zone of inhibition of 5.0. Significant differences were observed among the extracts, on well diffusion versus disc diffusion and ciprofloxacin (p<0.05). Despite the fact that methanol and aqueous extracts of the plants proved inhibitory actions to S. typhi more than the drug of choice in hospitals (Ciprofloxacin), Methanol extract of medicinal plants proved to inhibit the growth of S typhi strains more.
"SUSCEPTIBILITY OF Salmonella typhi TO MEDICINAL PLANT EXTRACTS COMPARED TO CIPROFLOXACIN", IJSDR - International Journal of Scientific Development and Research (www.IJSDR.org), ISSN:2455-2631, Vol.9, Issue 4, page no.1018 - 1023, April-2024, Available :https://ijsdr.org/papers/IJSDR2404145.pdf
Volume 9
Issue 4,
April-2024
Pages : 1018 - 1023
Paper Reg. ID: IJSDR_210277
Published Paper Id: IJSDR2404145
Downloads: 000347299
Research Area: Medical Science
Country: Bafoussam, West, Cameroon
ISSN: 2455-2631 | IMPACT FACTOR: 9.15 Calculated By Google Scholar | ESTD YEAR: 2016
An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 9.15 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator
Publisher: IJSDR(IJ Publication) Janvi Wave
