Formulation and Evaluation of Combinational Liposomes of Biochanin-A and Docetaxel for Breast Cancer Treatment

Ajay Kumar , Amrita Singh , Snehil Singh , Bhanu P. S. Sagar

Biochanin-A, cancer, cholesterol, cytotoxic potential, docetaxel, lipophilic, liposomes, nano-particles, phosphatidylcholine, sonication, soya lecithin

The solubility study of Biochanin A (BCA) and Docetaxel (DTX) indicated that both drugs were poorly soluble in water but shown the better solubility in HBSE buffer. So, the HBSE buffer was chosen as vehicle since sufficient amount of drug dissolve in it, which is necessary to maintain sink conditions. The partition coefficient of BCA and DTX was found to be 3.42 and 4.38 respectively. The values clearly indicated that both the drugs are lipophilic and soluble in lipids and so both the drugs have been added to the lipid part during preparation of liposome. Various formulations of the combination of BCA and DTX were prepared by sonication method with different ratios of soya lecithin and cholesterol at different sonication speed. Entrapment efficiency of the F6 formulation of Biochanin A was very high (70.0±0.58) then F6 formulation of Docetaxel (69.9±0.96) because of high sonication speed. The effect of the soya lecithin and cholesterol ratio in the lipid components of vesicles on the entrapment efficiency of lipophilic drugs, Biochanin A and Docetaxel, the efficiency was increased with sonication speed and decreased with the ratio of phosphatidylcholine. Particle size of liposomes was measured by particle size analyzer and best size of liposome formulation vehicle was 202±0.66 nm of formulations F6. MTT assay for selected formulations were performed and it was found that combination liposomes were having better cytotoxic potential than the individual drugs (separate drugs / drugs alone). The difference was statistically significant.

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"Formulation and Evaluation of Combinational Liposomes of Biochanin-A and Docetaxel for Breast Cancer Treatment", IJSDR - International Journal of Scientific Development and Research (www.IJSDR.org), ISSN:2455-2631, Vol.8, Issue 12, page no.530 - 543, December-2023, Available :https://ijsdr.org/papers/IJSDR2312071.pdf

Volume 8 Issue 12, December-2023

Pages : 530 - 543

Paper Reg. ID: IJSDR_209589

Published Paper Id: IJSDR2312071

Downloads: 000347217

Research Area: Pharmacy

Country: -, -, India

Published Paper PDF: https://ijsdr.org/papers/IJSDR2312071

Published Paper URL: https://ijsdr.org/viewpaperforall?paper=IJSDR2312071

ISSN: 2455-2631 | IMPACT FACTOR: 9.15 Calculated By Google Scholar | ESTD YEAR: 2016

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