Anti-hepatotoxic Activities of Combination of Wedelolactone and Berberine against Paracetamol / Atractyloside induced Hepatotoxicity

Muskan Saxena , Amrita Singh , Pratibha Singh , Mohd Zafar , Bhanu P. S. Sagar

Antihepatotoxic, atractyloside, berberine, glutathione, micrtomy, peroxidation, necrosis, paracetamol, wedelolactone.

Pharmacological studies were performed for anti-hepatotoxic activity of BB, WDL and combination of BB with WDl against PCM and ATR induced hepatotoxicity in albino rats. LFTs were assessed with biochemical kits of ALT, AST, AB, T-Prot, AKLP and T-Bil. PCM / ATR induced hepatoxicity increased hepatic biochemical parameters due to hepatic necrosis. Elevated LFTs levels were correlated with necrosis, infiltration, broad infiltration of lymphocytes and kupffer cells, degeneration of hepatocytes (histopathology of PCM and ATR induced dose dependent hepatic lesions or necrosis). Treatment with berberine (100 mg/kg), wedelolactone (100 mg/kg), berberine in combination with wedelolactone (50 mg/kg each) and standard silymarin (140mg/kg) controlled hepato-toxicities (recovered animals to normalcy) with significant alleviation of LFTs. Hepatoprotective properties of BB and WDL (either alone or in combination) significantly reduced lipid peroxidation. Combination showed a remarkable anti-hepatotoxic activity but comparatively lesser anti-hepatotoxic effects then Silymarin. Data was analyzed by ANOVA. Finally, it was found that BB in combination with WDL produced higher anti-hepatotoxic property then individual drug (BB / WDL) but lesser than Silymarin (Standard). Effects were induced by quenching of free radicals, attenuation depleted glutathione and membrane stabilization, inhibition of Phopholipase A2 and their use in combination is recommended for the treatment of DIH / DILI.

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"Anti-hepatotoxic Activities of Combination of Wedelolactone and Berberine against Paracetamol / Atractyloside induced Hepatotoxicity", IJSDR - International Journal of Scientific Development and Research (www.IJSDR.org), ISSN:2455-2631, Vol.8, Issue 9, page no.774 - 784, September-2023, Available :https://ijsdr.org/papers/IJSDR2309119.pdf

Volume 8 Issue 9, September-2023

Pages : 774 - 784

Paper Reg. ID: IJSDR_208661

Published Paper Id: IJSDR2309119

Downloads: 000347036

Research Area: Engineering

Country: -, -, India

Published Paper PDF: https://ijsdr.org/papers/IJSDR2309119

Published Paper URL: https://ijsdr.org/viewpaperforall?paper=IJSDR2309119

ISSN: 2455-2631 | IMPACT FACTOR: 9.15 Calculated By Google Scholar | ESTD YEAR: 2016

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