A Comprehensive overview of monoclonal antibodies production through various methods and applications
Daphne sherine.S
, Thirumalai.P , Poovarasan.P , Suhasini.G , Thilagavathi.A
Monoclonal antibodies; Hybridoma technology; Phage display method; Cancers; Graft rejections
In biomedical research, monoclonal antibodies are an indispensable tool. In 1975, Cesar Milstein and Georges J.F. Kohler developed the process for making monoclonal antibodies, for which they were awarded the 1945 Nobel Prize. Antigen injection to mice results in an immunological response, which is used to create monoclonal antibodies. The injected mouse is subsequently collected for its B cells. The utilisation of transgenic mice, the phage display platform, the hybridoma technologies, and the production of monoclonal antibodies from B-cells are all introduced in this overview. The types of MAbs created include humanised MAbs, which contain 10% mouse protein, chimeric MAbs, which contain 25% mouse protein, and murine MAbs, which contain 100% mouse protein. MAbs are utilised for cancer treatment, cancer diagnosis, and transplant rejection prevention. The synthesis of monoclonal antibodies from plants and the use of transgenic mice are both emerging strategies, but hybridoma technology is a more conventional approach. Polyclonal antibodies are not desired; monoclonal antibodies are. In comparison to hybridoma approach, the phage display method is a sophisticated technology that can be relied upon to create MAb at a faster rate. In-vitro and in-vivo procedures are used to continue both the hybridoma technique and the phage display method. Plastic cell culture equipment is required for the cell suspension culture technology, which can be applied to both small and big groups of people. The market value of MAbs in the medical industry is anticipated to increase significantly in the future.
"A Comprehensive overview of monoclonal antibodies production through various methods and applications", IJSDR - International Journal of Scientific Development and Research (www.IJSDR.org), ISSN:2455-2631, Vol.8, Issue 8, page no.1000 - 1021, August-2023, Available :https://ijsdr.org/papers/IJSDR2308148.pdf
Volume 8
Issue 8,
August-2023
Pages : 1000 - 1021
Paper Reg. ID: IJSDR_208214
Published Paper Id: IJSDR2308148
Downloads: 000347204
Research Area: Pharmacy
Country: krishnagiri, Tamil nadu, India
ISSN: 2455-2631 | IMPACT FACTOR: 9.15 Calculated By Google Scholar | ESTD YEAR: 2016
An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 9.15 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator
Publisher: IJSDR(IJ Publication) Janvi Wave
