Comparison between Sabouraud Dextrose Agar (SDA) and Potato Dextrose Agar (PDA) with Banana Peel (BP) for studying fungi in the air by settle-plate method.
Pritika Maji
, Abhijit Ghosh , Amit Banik , Atul raj
SDA, PDA with Banana Peel, Fungi, Settle Plate Method, Quality control, Environment, Indoor & Outdoor, Air sample.
The present study aimed to compare the efficacy of Sabouraud Dextrose Agar (SDA) and Potato Dextrose Agar (PDA) supplemented with Banana Peel (BP) as a substrate for studying airborne fungi using the settle-plate method. Airborne fungi pose significant health risks, and understanding their presence and diversity is crucial for effective control and prevention measures. Settle-plate sampling was employed to collect airborne fungal spores onto SDA and PDA media supplemented with BP. The BP addition was chosen due to its potential as a nutrient source for fungi, which may enhance their growth and promote their detection. The settle-plate method involved exposing the culture media to the air for a specified period, allowing the settling of airborne particles, including fungal spores. Settle-plates were utilized for each medium type, and the experiments were conducted in various indoor and outdoor environments with different levels of fungal contamination. After incubation, the colonies were enumerated and identified based on their morphological characteristics. The results revealed that both SDA and PDA supplemented with BP effectively supported the growth of airborne fungi. However, distinct differences were observed in terms of colony morphology and fungal diversity. SDA exhibited better selective properties for fungal growth, allowing the identification of a wide range of fungal species. On the other hand, PDA supplemented with BP exhibited a higher colony count, indicating enhanced fungal growth. Notably, the use of BP as a substrate provided additional advantages in terms of promoting fungal growth. The banana peels organic composition likely contributed to the nutritional enrichment of the culture media, leading to increased fungal colonization and improved detection sensitivity.
"Comparison between Sabouraud Dextrose Agar (SDA) and Potato Dextrose Agar (PDA) with Banana Peel (BP) for studying fungi in the air by settle-plate method.", IJSDR - International Journal of Scientific Development and Research (www.IJSDR.org), ISSN:2455-2631, Vol.8, Issue 7, page no.782 - 787, July-2023, Available :https://ijsdr.org/papers/IJSDR2307112.pdf
Volume 8
Issue 7,
July-2023
Pages : 782 - 787
Paper Reg. ID: IJSDR_207768
Published Paper Id: IJSDR2307112
Downloads: 000347254
Research Area: Life Sciences
Country: Purba Medinipur, West Bengal, India
DOI: http://doi.one/10.1729/Journal.36197
ISSN: 2455-2631 | IMPACT FACTOR: 9.15 Calculated By Google Scholar | ESTD YEAR: 2016
An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 9.15 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator
Publisher: IJSDR(IJ Publication) Janvi Wave