Troubleshooting in HPLC: A Review

Sayali A. Bhor , Priyanka P. Atnure , Manoj S. Charde , Rita D. Chakole , Shital T. Hajare

Key words: Troubleshooting, HPLC, etc.

The most effective method for many analytical procedures is chromatography. Compared to other chromatographic procedures, HPLC yields results with higher resolution and accuracy in less time. There are different types of HPLC from which reverse phase HPLC is mostly used because various components are polar in nature and easily separated. The approach to problem-solving used to identify and resolve HPLC system-related issues is known as HPLC troubleshooting. Problems with the HPLC System can come from a variety of causes. Define the issue first, then narrow down the source to identify the potential troublemaker. Troubleshooting HPLC instrumentation and separations require a fundamental understanding of how the instrument functions and how the separation works. This review is an attempt to describe overview of HPLC with its types and applications. Along with this, this article includes troubleshooting strategies and the process to overcome problems related to mobile phase, injector, detector, column and pumps. All these problems are due to pressure difference, leaks, chromatogram, baseline irregularities and smell, sight or sound related issues.

Published Paper   E-Certificate

"Troubleshooting in HPLC: A Review", IJSDR - International Journal of Scientific Development and Research (www.IJSDR.org), ISSN:2455-2631, Vol.8, Issue 6, page no.924 - 933, June-2023, Available :https://ijsdr.org/papers/IJSDR2306132.pdf

Volume 8 Issue 6, June-2023

Pages : 924 - 933

Paper Reg. ID: IJSDR_207223

Published Paper Id: IJSDR2306132

Downloads: 000347278

Research Area: Pharmacy

Country: SATARA, Not US or Canada, India

Published Paper PDF: https://ijsdr.org/papers/IJSDR2306132

Published Paper URL: https://ijsdr.org/viewpaperforall?paper=IJSDR2306132

ISSN: 2455-2631 | IMPACT FACTOR: 9.15 Calculated By Google Scholar | ESTD YEAR: 2016

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 9.15 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Publisher: IJSDR(IJ Publication) Janvi Wave

This work is licensed under a Creative Commons Attribution 4.0 International License and The Open Definition