OBSERVING THE DEGRADATIVE ABILITIES OF DIFFERENT BACTERIAL ISOLATES USING COMBINED METHOD (DCIP, TURBIDITY, PH AND SPECTROPHOTOMETRIC APPROACH)
Okorhi-Damisa F.B
, Tudararo -Aherobo, Laurelta
TPH, TURBIDITY, SPECTROPHOTOMETER
The petroleum industry supplies a substantial quantity of world’s energy demands in addition to popular petro-chemical intermediates required for production of extensive range of goods like solvents, dye stuffs, pharmaceuticals, polymers, and new chemicals etc. All these tend to generate environmental pollution when discharged in the environment; however the aim of this study is to ascertain the degradative abilities of the organisms isolated from the impacted site by monitoring their degradative abilities, the hydrocarbon-degrading potential of eight bacterial isolates were monitored using spectrophotometer, turbidity and redox indicator dye 2,6-dichlorophenolindophenol (DCIP).These isolates were identified based on their macroscopic, microscopic, biochemical and molecular characteristics, they were identified as:Bacillus thuringiensis, Bacillus velezensis, Lysinibacillus fusiformis, Citrobacter specie, Poor Sequence quality, Stenotrophomonas specie,and Lysinibacillus pakistanensis. The screening was carried out for a period of 24-360 hours monitoring their TPH, Turbidity and pH with various control alongside, however the results obtained from the study shows that the pH were between 5.11± to 6.11± and control having the pH of 5.29± and 6.59 ± respectively, while the TPH were between the range of 1204.88mg/l and 136.72mg/l and control recorded the values within 1204.88mg/l and 644.24mg/l while for turbidity the values were between 1109.4NTU and 2532.0NTU and the values of the control were within 640.02NTU and 1215.0NTU . Thus considering the easiness and conciseness of sample preparation, high through put screening using Turbidometric and spectrophotometric approach could be considered as an efficient, accurate and rapid method of quantifying the level of hydrocarbon present in a sample. This study shows that the quantity of hydrocarbon utilizing bacterial produced by the strains has the potential to biodegrade and cleans up any toxic substance present in the environment.
"OBSERVING THE DEGRADATIVE ABILITIES OF DIFFERENT BACTERIAL ISOLATES USING COMBINED METHOD (DCIP, TURBIDITY, PH AND SPECTROPHOTOMETRIC APPROACH)", IJSDR - International Journal of Scientific Development and Research (www.IJSDR.org), ISSN:2455-2631, Vol.8, Issue 3, page no.543 - 552, March-2023, Available :https://ijsdr.org/papers/IJSDR2303086.pdf
Volume 8
Issue 3,
March-2023
Pages : 543 - 552
Paper Reg. ID: IJSDR_204370
Published Paper Id: IJSDR2303086
Downloads: 000347415
Research Area: Life Sciences
Country: Delta state, Delta, Nigeria
ISSN: 2455-2631 | IMPACT FACTOR: 9.15 Calculated By Google Scholar | ESTD YEAR: 2016
An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 9.15 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator
Publisher: IJSDR(IJ Publication) Janvi Wave
