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INTERNATIONAL JOURNAL OF SCIENTIFIC DEVELOPMENT AND RESEARCH International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2455-2631 | Impact factor: 8.15 | ESTD Year: 2016 open access , Peer-reviewed, and Refereed Journals, Impact factor 8.15 |
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| Paper Title: | OBSERVING THE DEGRADATIVE ABILITIES OF DIFFERENT BACTERIAL ISOLATES USING COMBINED METHOD (DCIP, TURBIDITY, PH AND SPECTROPHOTOMETRIC APPROACH) |
| Authors Name: | Okorhi-Damisa F.B , Tudararo -Aherobo, Laurelta |
| Unique Id: | IJSDR2303086 |
| Published In: | Volume 8 Issue 3, March-2023 |
| Abstract: | The petroleum industry supplies a substantial quantity of world’s energy demands in addition to popular petro-chemical intermediates required for production of extensive range of goods like solvents, dye stuffs, pharmaceuticals, polymers, and new chemicals etc. All these tend to generate environmental pollution when discharged in the environment; however the aim of this study is to ascertain the degradative abilities of the organisms isolated from the impacted site by monitoring their degradative abilities, the hydrocarbon-degrading potential of eight bacterial isolates were monitored using spectrophotometer, turbidity and redox indicator dye 2,6-dichlorophenolindophenol (DCIP).These isolates were identified based on their macroscopic, microscopic, biochemical and molecular characteristics, they were identified as:Bacillus thuringiensis, Bacillus velezensis, Lysinibacillus fusiformis, Citrobacter specie, Poor Sequence quality, Stenotrophomonas specie,and Lysinibacillus pakistanensis. The screening was carried out for a period of 24-360 hours monitoring their TPH, Turbidity and pH with various control alongside, however the results obtained from the study shows that the pH were between 5.11± to 6.11± and control having the pH of 5.29± and 6.59 ± respectively, while the TPH were between the range of 1204.88mg/l and 136.72mg/l and control recorded the values within 1204.88mg/l and 644.24mg/l while for turbidity the values were between 1109.4NTU and 2532.0NTU and the values of the control were within 640.02NTU and 1215.0NTU . Thus considering the easiness and conciseness of sample preparation, high through put screening using Turbidometric and spectrophotometric approach could be considered as an efficient, accurate and rapid method of quantifying the level of hydrocarbon present in a sample. This study shows that the quantity of hydrocarbon utilizing bacterial produced by the strains has the potential to biodegrade and cleans up any toxic substance present in the environment. |
| Keywords: | TPH, TURBIDITY, SPECTROPHOTOMETER |
| Cite Article: | "OBSERVING THE DEGRADATIVE ABILITIES OF DIFFERENT BACTERIAL ISOLATES USING COMBINED METHOD (DCIP, TURBIDITY, PH AND SPECTROPHOTOMETRIC APPROACH)", International Journal of Science & Engineering Development Research (www.ijsdr.org), ISSN:2455-2631, Vol.8, Issue 3, page no.543 - 552, March-2023, Available :http://www.ijsdr.org/papers/IJSDR2303086.pdf |
| Downloads: | 000337066 |
| Publication Details: | Published Paper ID: IJSDR2303086 Registration ID:204370 Published In: Volume 8 Issue 3, March-2023 DOI (Digital Object Identifier): Page No: 543 - 552 Publisher: IJSDR | www.ijsdr.org ISSN Number: 2455-2631 |
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