Method validation for hydroxycitric acid lactone from garcinia indica by hplc methods

Dilip singh , Shobha yadav , Sandeep singh yadav , Shubham pandey

Garcinia indica; (−)-Hydroxycitric acid; Antiobesity.

A simple HPLC (high performance liquid chromatography) method for the validation of HCA in Garcinia indica has been developed. HCA is major acid component was shown to be potent inhibitor of alpha amylase and alpha glucosidase leading to reduction of carbohydrate metabolism, results in weight loss.HCA inhibits ATP–citrate lyase, a key enzyme responsible for promoting fat synthesis. As a result of this inhibition, energy is di-verted for the production of glycogen in liver and muscles, instead of fat synthesis. Since higher glycogen levels signal satiety to the brain, HCA suppresses appetite. As a direct result of ATP–citrate lyase inhibition, malonyl-CoA is not produced, which indirectly helps in burning the fat. The study of the mechanism of action of HCAL would be difficult for the reason that it exists in equilibrium with HCA under aqueous/acidic conditions. HCAL shows better activity in terms of appetite suppression and reduction in weight gain and this may be due to continuous conversion of HCAL into HCA as it gets utilized by the body. HCAL may thus act as a pro- drug. Once HCAL gets converted into HCA it follows the same mechanism of action as HCA in reducing body weight gain and appetite suppression.

Published Paper   E-Certificate

"Method validation for hydroxycitric acid lactone from garcinia indica by hplc methods", IJSDR - International Journal of Scientific Development and Research (www.IJSDR.org), ISSN:2455-2631, Vol.8, Issue 2, page no.52 - 55, February-2023, Available :https://ijsdr.org/papers/IJSDR2302010.pdf

Volume 8 Issue 2, February-2023

Pages : 52 - 55

Paper Reg. ID: IJSDR_203749

Published Paper Id: IJSDR2302010

Downloads: 000347320

Research Area: Pharmacy

Country: Varanasi, Uttar pradesh, India

Published Paper PDF: https://ijsdr.org/papers/IJSDR2302010

Published Paper URL: https://ijsdr.org/viewpaperforall?paper=IJSDR2302010

ISSN: 2455-2631 | IMPACT FACTOR: 9.15 Calculated By Google Scholar | ESTD YEAR: 2016

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 9.15 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Publisher: IJSDR(IJ Publication) Janvi Wave

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