Study on the presence of bacterial isolates with the potential to produce antimicrobial peptides in soil samples in and around Mysore city, India

Shanthala Sundar , N Lakshmidevi

Bacillus siamensis, antimicrobial peptides, soil bacteria, market, antagonism, human pathogens

The present study aims to screen soil samples collected from market regions in and around Mysore district, India, for the presence of antimicrobial peptides-producing bacteria. Totally 30 samples were subjected to isolation using the standard serial dilution technique. Morphological characterization of the isolated presumptive cultures by Gram’s staining method confirmed the presence of both Gram-positive and Gram-negative bacteria in the samples screened, the former being more in number. Primary screening of the isolates using the perpendicular streaking method resulted in an encounter of four isolates with antibacterial activity viz. AGS7, AKS9, AZS12, and BPS26. Among them, processed culture supernatant of AZS12 strain demonstrated broad-spectrum antibacterial activity against clinically important bacterial pathogens namely Klebsiella pneumoniae, Pseudomonas aeruginosa, Proteus mirabilis, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus mutans, and Salmonella enterica ser. paratyphi. A maximum zone of inhibition of 22 mm was recorded against K. pneumoniae and Streptococcus mutans at a 50 µl concentration of cell-free culture supernatant of AZS12. This secondary screening validated that AZS12 was an antimicrobial peptide-producing bacterial isolate. Biochemical characterization using a panel of tests indicated that AZS12 belonged to the genus of Bacillus and 16S rRNA gene sequence analysis revealed that the isolate was a strain of B. siamensis. The sequence was deposited in GenBank under the accession number ON197155. Further, phylogenetic analysis of the obtained 16S rRNA sequence with the other members of Bacillus spp. indicated that AZS12 was a novel strain of B. siamensis. Finally, the culture was deposited in National Centre for Cell Science (NCCS).

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"Study on the presence of bacterial isolates with the potential to produce antimicrobial peptides in soil samples in and around Mysore city, India", IJSDR - International Journal of Scientific Development and Research (www.IJSDR.org), ISSN:2455-2631, Vol.7, Issue 12, page no.1076 - 1083, December-2022, Available :https://ijsdr.org/papers/IJSDR2212172.pdf

Volume 7 Issue 12, December-2022

Pages : 1076 - 1083

Paper Reg. ID: IJSDR_203264

Published Paper Id: IJSDR2212172

Downloads: 000347235

Research Area: Life Sciences 

Country: Mysuru, Karnataka, India

Published Paper PDF: https://ijsdr.org/papers/IJSDR2212172

Published Paper URL: https://ijsdr.org/viewpaperforall?paper=IJSDR2212172

ISSN: 2455-2631 | IMPACT FACTOR: 9.15 Calculated By Google Scholar | ESTD YEAR: 2016

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 9.15 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Publisher: IJSDR(IJ Publication) Janvi Wave

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