A Validated RP-HPLC Method for Analysis of Chloramphenicol Residue in Pasteurized Milk Sample in Bangladesh
Hasib Khan Shomudro
, Mithila Khatun , Sherejad Sanam
Key Words: Chloramphenicol, Pasteurized milk, RP-HPLC, Robustness, Mobile phase, Quantification.
Abstract: The present study of this research was to develop a useful HPLC sensitive method to validate and determine the presence of trace amount of chloramphenicol in pasteurized milk. Method was developed by using Waters C18 (150×4.6, 5µm). The mobile phase was isocratic, composed of phosphate buffer pH 6.8 and acetonitrile (20:80), pumped at a flow rate of 1 mL/minute, and wavelength was 270 nm. The method was validated with deproteinized milk sample using Trichloro acetic acid and acetonitrile for system suitability, accuracy, linearity, precision study, robustness, limit of detection, and limit of quantification. Finally, determination of chloramphenicol of tested samples were observed. Trace amount of chloramphenicol in Bangladesh is rare. The findings of this research may aid in the investigation of the existence of trace amounts of chloramphenicol in pasteurized milk samples, as well as the fact that the presence of antibiotics in foods poses a serious hazard to health of human. From this study, we can define that chloramphenicol presence may not found, but if it was present in a minimal amount that may have an alarming issue but range was very minimal.
"A Validated RP-HPLC Method for Analysis of Chloramphenicol Residue in Pasteurized Milk Sample in Bangladesh", IJSDR - International Journal of Scientific Development and Research (www.IJSDR.org), ISSN:2455-2631, Vol.7, Issue 9, page no.33 - 40, September-2022, Available :https://ijsdr.org/papers/IJSDR2209005.pdf
Volume 7
Issue 9,
September-2022
Pages : 33 - 40
Paper Reg. ID: IJSDR_201391
Published Paper Id: IJSDR2209005
Downloads: 000347347
Research Area: Health Science
Country: Dhaka, Dhaka, Bangladesh
ISSN: 2455-2631 | IMPACT FACTOR: 9.15 Calculated By Google Scholar | ESTD YEAR: 2016
An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 9.15 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator
Publisher: IJSDR(IJ Publication) Janvi Wave
