Effect of AgNO3 nanoparticle on bull spermatozoa kinetic parameters to optimize best supplementation concentration without adverse effects

Arushi Kanwar , Meenakshi Virmani , Ankit Magotra , Sandeep Kumar , Sant Lal

Sperm, silver, nanoparticle, CASA

The objective of this study was to determine the antibacterial effect of silver nitrate nanoparticles (AgNO3) and its potential effect on cattle bull semen as an alternative to replace the conventional antibiotics to reduce antibiotic resistance that arises due to indiscriminate use in animals. The antibacterial effect of AgNO3 NPs prepared by chemical reduction method was determined by disc diffusion method and it was found that antibacterial effect of AgNO3 NP increased in a dose dependent manner. The prepared NPs were characterized by DLS (Dynamic Light Scattering), TEM (Transmission Electron Microscope), SEM (Scanning Electron Microscopy). Effect of different concentrations of NP (250, 500, 750, 1000, 1500 µg/ml) on CASA motility parameters of spermatozoa was studied at 0, 15 and 30 minute interval post treatment. Various kinetic parameters like DAP, DCL, DSL, BCF, VAP, VCL showed improvement in nanosupplemented samples at concentration of 250 µg/ml compared to control group but declined at higher concentration of NP. The percent motile and progressive motile sperms at 0 and 15 min incubation in groups 1 and 2 supplemented with 250 and 500 µg/ml NPs were comparable to that of control group. In conclusion, AgNO3 NPs showed antibacterial activity and can be used to replace antibiotics used in semen but in doses lower then 250 µg/ml without adversely affecting CASA motility parameters of spermatozoa.

Published Paper   E-Certificate

"Effect of AgNO3 nanoparticle on bull spermatozoa kinetic parameters to optimize best supplementation concentration without adverse effects", IJSDR - International Journal of Scientific Development and Research (www.IJSDR.org), ISSN:2455-2631, Vol.7, Issue 8, page no.847 - 856, August-2022, Available :https://ijsdr.org/papers/IJSDR2208125.pdf

Volume 7 Issue 8, August-2022

Pages : 847 - 856

Paper Reg. ID: IJSDR_201407

Published Paper Id: IJSDR2208125

Downloads: 000347173

Research Area: Health Science 

Country: HAMIRPUR, Himachal Pradesh, India

Published Paper PDF: https://ijsdr.org/papers/IJSDR2208125

Published Paper URL: https://ijsdr.org/viewpaperforall?paper=IJSDR2208125

ISSN: 2455-2631 | IMPACT FACTOR: 9.15 Calculated By Google Scholar | ESTD YEAR: 2016

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 9.15 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Publisher: IJSDR(IJ Publication) Janvi Wave

This work is licensed under a Creative Commons Attribution 4.0 International License and The Open Definition