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INTERNATIONAL JOURNAL OF SCIENTIFIC DEVELOPMENT AND RESEARCH International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2455-2631 | Impact factor: 8.15 | ESTD Year: 2016 open access , Peer-reviewed, and Refereed Journals, Impact factor 8.15 |
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| Paper Title: | Effect of AgNO3 nanoparticle on bull spermatozoa kinetic parameters to optimize best supplementation concentration without adverse effects |
| Authors Name: | Arushi Kanwar , Meenakshi Virmani , Ankit Magotra , Sandeep Kumar , Sant Lal |
| Unique Id: | IJSDR2208125 |
| Published In: | Volume 7 Issue 8, August-2022 |
| Abstract: | The objective of this study was to determine the antibacterial effect of silver nitrate nanoparticles (AgNO3) and its potential effect on cattle bull semen as an alternative to replace the conventional antibiotics to reduce antibiotic resistance that arises due to indiscriminate use in animals. The antibacterial effect of AgNO3 NPs prepared by chemical reduction method was determined by disc diffusion method and it was found that antibacterial effect of AgNO3 NP increased in a dose dependent manner. The prepared NPs were characterized by DLS (Dynamic Light Scattering), TEM (Transmission Electron Microscope), SEM (Scanning Electron Microscopy). Effect of different concentrations of NP (250, 500, 750, 1000, 1500 µg/ml) on CASA motility parameters of spermatozoa was studied at 0, 15 and 30 minute interval post treatment. Various kinetic parameters like DAP, DCL, DSL, BCF, VAP, VCL showed improvement in nanosupplemented samples at concentration of 250 µg/ml compared to control group but declined at higher concentration of NP. The percent motile and progressive motile sperms at 0 and 15 min incubation in groups 1 and 2 supplemented with 250 and 500 µg/ml NPs were comparable to that of control group. In conclusion, AgNO3 NPs showed antibacterial activity and can be used to replace antibiotics used in semen but in doses lower then 250 µg/ml without adversely affecting CASA motility parameters of spermatozoa. |
| Keywords: | Sperm, silver, nanoparticle, CASA |
| Cite Article: | "Effect of AgNO3 nanoparticle on bull spermatozoa kinetic parameters to optimize best supplementation concentration without adverse effects", International Journal of Science & Engineering Development Research (www.ijsdr.org), ISSN:2455-2631, Vol.7, Issue 8, page no.847 - 856, August-2022, Available :http://www.ijsdr.org/papers/IJSDR2208125.pdf |
| Downloads: | 000337068 |
| Publication Details: | Published Paper ID: IJSDR2208125 Registration ID:201407 Published In: Volume 7 Issue 8, August-2022 DOI (Digital Object Identifier): Page No: 847 - 856 Publisher: IJSDR | www.ijsdr.org ISSN Number: 2455-2631 |
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