OPTIMIZATION AND PURIFICATION OF INDOLE ACETIC ACID (IAA) PRODUCED BY RHIZOSPHERIC PSUEOMONAS SPP.

Diwate Ankita Ashok , Shaikh Sajid , Khatal Mayuri Kailas

IAA

Indole-3-acetic acid (IAA, 3-IAA) is the most common, naturally occurring, plant hormone of the auxin class. Microorganism have ability to produce IAA. The present work deals with isolation, characterization, optimization and purification of Indole acetic acid (IAA) producing bacteria form rhizospheric soil sample from wheat plant. The IAA producing bacteria were isolated, screened and purified on Nutrient agar with tryptophan plates. Screening resulted in selection of bacterial isolate grow on the plates were they able to utilize tryptophan and produce the IAA. The morphological characterization showed the bacteria to be a Gram negative, motile, short rod-shaped bacterium. The biochemical tests indicated that the bacteria belonged to the Pseudomonas Spp. (According to Bergey’s Manual of Determinative Bacteriology). The bacterial isolate was then subjected to IAA production, which was further optimized with various parameters like incubation temperature, pH, salt concentration and also with different carbon and nitrogen sources.

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"OPTIMIZATION AND PURIFICATION OF INDOLE ACETIC ACID (IAA) PRODUCED BY RHIZOSPHERIC PSUEOMONAS SPP.", IJSDR - International Journal of Scientific Development and Research (www.IJSDR.org), ISSN:2455-2631, Vol.7, Issue 6, page no.194 - 204, June-2022, Available :https://ijsdr.org/papers/IJSDR2206031.pdf

Volume 7 Issue 6, June-2022

Pages : 194 - 204

Paper Reg. ID: IJSDR_200519

Published Paper Id: IJSDR2206031

Downloads: 000347039

Research Area: Life Sciences 

Country: Ahmednagar, Maharashtra, India

Published Paper PDF: https://ijsdr.org/papers/IJSDR2206031

Published Paper URL: https://ijsdr.org/viewpaperforall?paper=IJSDR2206031

ISSN: 2455-2631 | IMPACT FACTOR: 9.15 Calculated By Google Scholar | ESTD YEAR: 2016

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 9.15 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Publisher: IJSDR(IJ Publication) Janvi Wave

This work is licensed under a Creative Commons Attribution 4.0 International License and The Open Definition