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ISSN Approved Journal No: 2455-2631 | Impact factor: 8.15 | ESTD Year: 2016
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Issue: June 2022

Volume 7 | Issue 6

Impact factor: 8.15

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Paper Title: OPTIMIZATION AND PURIFICATION OF INDOLE ACETIC ACID (IAA) PRODUCED BY RHIZOSPHERIC PSUEOMONAS SPP.
Authors Name: Diwate Ankita Ashok , Shaikh Sajid , Khatal Mayuri Kailas
Unique Id: IJSDR2206031
Published In: Volume 7 Issue 6, June-2022
Abstract: Indole-3-acetic acid (IAA, 3-IAA) is the most common, naturally occurring, plant hormone of the auxin class. Microorganism have ability to produce IAA. The present work deals with isolation, characterization, optimization and purification of Indole acetic acid (IAA) producing bacteria form rhizospheric soil sample from wheat plant. The IAA producing bacteria were isolated, screened and purified on Nutrient agar with tryptophan plates. Screening resulted in selection of bacterial isolate grow on the plates were they able to utilize tryptophan and produce the IAA. The morphological characterization showed the bacteria to be a Gram negative, motile, short rod-shaped bacterium. The biochemical tests indicated that the bacteria belonged to the Pseudomonas Spp. (According to Bergey’s Manual of Determinative Bacteriology). The bacterial isolate was then subjected to IAA production, which was further optimized with various parameters like incubation temperature, pH, salt concentration and also with different carbon and nitrogen sources.
Keywords: IAA
Cite Article: "OPTIMIZATION AND PURIFICATION OF INDOLE ACETIC ACID (IAA) PRODUCED BY RHIZOSPHERIC PSUEOMONAS SPP.", International Journal of Science & Engineering Development Research (www.ijsdr.org), ISSN:2455-2631, Vol.7, Issue 6, page no.194 - 204, June-2022, Available :http://www.ijsdr.org/papers/IJSDR2206031.pdf
Downloads: 00096794
Publication Details: Published Paper ID: IJSDR2206031
Registration ID:200519
Published In: Volume 7 Issue 6, June-2022
DOI (Digital Object Identifier):
Page No: 194 - 204
Publisher: IJSDR | www.ijsdr.org
ISSN Number: 2455-2631

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