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INTERNATIONAL JOURNAL OF SCIENTIFIC DEVELOPMENT AND RESEARCH International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2455-2631 | Impact factor: 8.15 | ESTD Year: 2016 open access , Peer-reviewed, and Refereed Journals, Impact factor 8.15 |
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| Paper Title: | OPTIMIZATION AND PURIFICATION OF INDOLE ACETIC ACID (IAA) PRODUCED BY RHIZOSPHERIC PSUEOMONAS SPP. |
| Authors Name: | Diwate Ankita Ashok , Shaikh Sajid , Khatal Mayuri Kailas |
| Unique Id: | IJSDR2206031 |
| Published In: | Volume 7 Issue 6, June-2022 |
| Abstract: | Indole-3-acetic acid (IAA, 3-IAA) is the most common, naturally occurring, plant hormone of the auxin class. Microorganism have ability to produce IAA. The present work deals with isolation, characterization, optimization and purification of Indole acetic acid (IAA) producing bacteria form rhizospheric soil sample from wheat plant. The IAA producing bacteria were isolated, screened and purified on Nutrient agar with tryptophan plates. Screening resulted in selection of bacterial isolate grow on the plates were they able to utilize tryptophan and produce the IAA. The morphological characterization showed the bacteria to be a Gram negative, motile, short rod-shaped bacterium. The biochemical tests indicated that the bacteria belonged to the Pseudomonas Spp. (According to Bergey’s Manual of Determinative Bacteriology). The bacterial isolate was then subjected to IAA production, which was further optimized with various parameters like incubation temperature, pH, salt concentration and also with different carbon and nitrogen sources. |
| Keywords: | IAA |
| Cite Article: | "OPTIMIZATION AND PURIFICATION OF INDOLE ACETIC ACID (IAA) PRODUCED BY RHIZOSPHERIC PSUEOMONAS SPP.", International Journal of Science & Engineering Development Research (www.ijsdr.org), ISSN:2455-2631, Vol.7, Issue 6, page no.194 - 204, June-2022, Available :http://www.ijsdr.org/papers/IJSDR2206031.pdf |
| Downloads: | 000346990 |
| Publication Details: | Published Paper ID: IJSDR2206031 Registration ID:200519 Published In: Volume 7 Issue 6, June-2022 DOI (Digital Object Identifier): Page No: 194 - 204 Publisher: IJSDR | www.ijsdr.org ISSN Number: 2455-2631 |
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