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INTERNATIONAL JOURNAL OF SCIENTIFIC DEVELOPMENT AND RESEARCH International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2455-2631 | Impact factor: 8.15 | ESTD Year: 2016 open access , Peer-reviewed, and Refereed Journals, Impact factor 8.15 |
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| Paper Title: | Isolation of Methylene blue dye degrading bacteria from effluent, screening and evaluation of their dye decolorization activity |
| Authors Name: | Pranali Rajaram Jadhav , Dr. Kishori Swapnil Tarfe |
| Unique Id: | IJSDR1908035 |
| Published In: | Volume 4 Issue 8, August-2019 |
| Abstract: | Rapid industrialization has led to accumulation of various toxic elements that harm the environment and ultimately affect the human lives. Synthetic dyes are widely used because of cost effective synthesis, high stability to light, temperature etc. as compared to natural dyes. Textile dye effluents generally consist of toxic synthetic dyes which consist of 50%-70% of azo dyes. Bioremediation has become a key to deal with hazardous pollutants. Various bacteria such as Bacillus subtilis, Aeromonas hydrophila and Bacillus cereus, fungi & actinomycetes have been found to possess dye decolorizing activity. Bacteria from dye contaminated effluent may possess dye decolorizing ability due to their adaptation to extreme environment conditions. Media consisting of Methylene blue at different concentrations ranging from 0.005%, 0.01% and 1 % were used in order to isolate dye degrading organisms from effluent sample. Colony characteristics of organisms obtained from medium containing Methylene blue were studied and biochemical tests i.e. IMViC, TSI were performed for identification. Dye decolorization assay was performed colorimetrically at 615 nm for concentrations of Methylene blue ranging from 0.005%, 0.01% and 1% every 24 hours. The percentage of dye decolorization activity was found to be 92%, 51% and 0.03% for Methylene blue for concentrations ranging from 0.005%, 0.01% and 1% respectively. The probable isolated organism from Methylene blue i.e. Providencia sp. was found to possess the ability to decolorize Methylene blue at lower concentration. The probable isolates obtained needs to be further investigated regarding various factors such as media composition affecting dye degradation & mechanism of dye degrading activity. |
| Keywords: | Synthetic dyes, Bioremediation, Dye decolorization assay, Providencia sp., Methylene blue. |
| Cite Article: | "Isolation of Methylene blue dye degrading bacteria from effluent, screening and evaluation of their dye decolorization activity", International Journal of Science & Engineering Development Research (www.ijsdr.org), ISSN:2455-2631, Vol.4, Issue 8, page no.224 - 230, August-2019, Available :http://www.ijsdr.org/papers/IJSDR1908035.pdf |
| Downloads: | 000337070 |
| Publication Details: | Published Paper ID: IJSDR1908035 Registration ID:190886 Published In: Volume 4 Issue 8, August-2019 DOI (Digital Object Identifier): Page No: 224 - 230 Publisher: IJSDR | www.ijsdr.org ISSN Number: 2455-2631 |
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