Isolation of Methylene blue dye degrading bacteria from effluent, screening and evaluation of their dye decolorization activity

Pranali Rajaram Jadhav , Dr. Kishori Swapnil Tarfe

Synthetic dyes, Bioremediation, Dye decolorization assay, Providencia sp., Methylene blue.

Rapid industrialization has led to accumulation of various toxic elements that harm the environment and ultimately affect the human lives. Synthetic dyes are widely used because of cost effective synthesis, high stability to light, temperature etc. as compared to natural dyes. Textile dye effluents generally consist of toxic synthetic dyes which consist of 50%-70% of azo dyes. Bioremediation has become a key to deal with hazardous pollutants. Various bacteria such as Bacillus subtilis, Aeromonas hydrophila and Bacillus cereus, fungi & actinomycetes have been found to possess dye decolorizing activity. Bacteria from dye contaminated effluent may possess dye decolorizing ability due to their adaptation to extreme environment conditions. Media consisting of Methylene blue at different concentrations ranging from 0.005%, 0.01% and 1 % were used in order to isolate dye degrading organisms from effluent sample. Colony characteristics of organisms obtained from medium containing Methylene blue were studied and biochemical tests i.e. IMViC, TSI were performed for identification. Dye decolorization assay was performed colorimetrically at 615 nm for concentrations of Methylene blue ranging from 0.005%, 0.01% and 1% every 24 hours. The percentage of dye decolorization activity was found to be 92%, 51% and 0.03% for Methylene blue for concentrations ranging from 0.005%, 0.01% and 1% respectively. The probable isolated organism from Methylene blue i.e. Providencia sp. was found to possess the ability to decolorize Methylene blue at lower concentration. The probable isolates obtained needs to be further investigated regarding various factors such as media composition affecting dye degradation & mechanism of dye degrading activity.

Published Paper   E-Certificate

"Isolation of Methylene blue dye degrading bacteria from effluent, screening and evaluation of their dye decolorization activity", IJSDR - International Journal of Scientific Development and Research (www.IJSDR.org), ISSN:2455-2631, Vol.4, Issue 8, page no.224 - 230, August-2019, Available :https://ijsdr.org/papers/IJSDR1908035.pdf

Volume 4 Issue 8, August-2019

Pages : 224 - 230

Paper Reg. ID: IJSDR_190886

Published Paper Id: IJSDR1908035

Downloads: 000347220

Research Area: Biological Science

Country: Badlapur / Thane, Maharashtra, India

Published Paper PDF: https://ijsdr.org/papers/IJSDR1908035

Published Paper URL: https://ijsdr.org/viewpaperforall?paper=IJSDR1908035

ISSN: 2455-2631 | IMPACT FACTOR: 9.15 Calculated By Google Scholar | ESTD YEAR: 2016

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 9.15 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Publisher: IJSDR(IJ Publication) Janvi Wave

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