INTERNATIONAL JOURNAL OF SCIENTIFIC DEVELOPMENT AND RESEARCH International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2455-2631 | Impact factor: 8.15 | ESTD Year: 2016
open access , Peer-reviewed, and Refereed Journals, Impact factor 8.15
Two growth regulators 2,4-D and Kn and three Indian wheat cultivars were used in the current research to evaluate the most suitable concentrations for callus induction, in vitro organogenesis. Furthermore, Cytokinin (BAP) and Auxin (α-NAA) along with calcium for the same cultivars were used for it multiplication of shoots in vitro organogenesis of the three wheat cultivars (Triticum aestivum L.); namely, “Raj-3765, Up-2425 and Hd-2932''. Different combination of BAP and α-NAA along with calcium (CaCl2.2H2O) 200-1200mg/l were used for organogenesis and the best results were recorded with BAP (2.0mg/L) + α-NAA (0.5mg/L) with (CaCl2.2H2O) 600mg/l. Later on culture were transfer for its elongation and multiplication.
Keywords:
BAP, Calcium, Callogenesis, PGR, Triticum aestivum L.
Cite Article:
"Effect of Calcium with Plant growth regulators on in vitro callogenesis and multiplication of cultivars of wheat (Raj-3765, Up-2425 and Hd-2932)", International Journal of Science & Engineering Development Research (www.ijsdr.org), ISSN:2455-2631, Vol.4, Issue 3, page no.365 - 371, March-2019, Available :http://www.ijsdr.org/papers/IJSDR1903062.pdf
Downloads:
000337077
Publication Details:
Published Paper ID: IJSDR1903062
Registration ID:190256
Published In: Volume 4 Issue 3, March-2019
DOI (Digital Object Identifier):
Page No: 365 - 371
Publisher: IJSDR | www.ijsdr.org
ISSN Number: 2455-2631
Facebook Twitter Instagram LinkedIn