PRELIMINARY PHYTOCHEMICAL ANALYSIS, IN VITRO ANTI-ARTHRITIC AND ANTI-INFLAMMATORY ACTIVITY OF AMMANNIA BACCIFEARA
Preliminary Phytochemical Analysis, In Vitro Anti-Arthritic, Anti-Inflammatory Activity, Ammannia Baccifeara
The phytochemical screening of the hydroalcoholic extract of Ammannia baccifera. (Leaf) powder revealed the presence of alkaloids, carbohydrates, sterols, saponins, tannins and phenolic compound, flavonoids, protein and free aminoacid, terpenoids, mucilage, betacyanin, quinone, phlobatannins, carotenoids. It shows the absence of anthraquinone glycosides, cardiac glycoside, fixed oil, anthocyanin, lecoanthocyanin, emodin, gum, resins, volatile oil. A study has reported that the flavonoids exert membrane stabilizing effect on lysosomes both invitro and invivo in experimental animals. Another report has suggested that tannins and saponins have the ability to bind cations and other biomolecules and are able to stabilize the erythrocyte membrane. HAEAB extract is highly potent on human erythrocyte and thus adequately protecting it against heat and hypotonicity induced lysis. The inhibitory concentration (IC50) of Ammannia baccifera. (Leaf) in HRBC membrane stabilization study is found to be 69µg/ml in comparison with diclofenac sodium 57µg/ml. It showed mild anti-inflammatory activity. The phytochemical analysis showed that the HRBC has flavonoids and tannins. Hence the HRBC membrane stabilizing capacity may be due to the presence of the above mentioned constituents which will prevent the oxidation of haemoglobin and also due to its antioxidant property. The principle involved is the inhibition of protein denaturation. Denaturation of protein was found to be one of the causes of rheumatoid arthritis. In rheumatoid arthritis, the production of autoantigen may be due to protein denaturation which involves the alteration of electrostatic hydrogen, hydrophobic and disulphide bonding. The protein used in this study is bovine serum albumin. Denaturation of protein is carried out by heating. The aim of this activity is to inhibit denaturation and to exhibit protective effect against rheumatoid arthritis. The inhibitory concentration (IC50) of Ammannia baccifera. (Leaf) in Protein denaturation is found to be 17µg/ml in comparison with diclofenac sodium 14µg/ml. It showed moderate anti-arthritic activity. The inhibition of protein denaturation by HAEAB may be due to the presence of phenolic compounds, flavonoids and tannins.
"PRELIMINARY PHYTOCHEMICAL ANALYSIS, IN VITRO ANTI-ARTHRITIC AND ANTI-INFLAMMATORY ACTIVITY OF AMMANNIA BACCIFEARA", IJSDR - International Journal of Scientific Development and Research (www.IJSDR.org), ISSN:2455-2631, Vol.9, Issue 3, page no.556 - 565, March-2024, Available :https://ijsdr.org/papers/IJSDR2403084.pdf
Volume 9
Issue 3,
March-2024
Pages : 556 - 565
Paper Reg. ID: IJSDR_210421
Published Paper Id: IJSDR2403084
Downloads: 000347074
Research Area: Pharmacy
Country: warangal, TELANGANA, India
ISSN: 2455-2631 | IMPACT FACTOR: 9.15 Calculated By Google Scholar | ESTD YEAR: 2016
An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 9.15 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator
Publisher: IJSDR(IJ Publication) Janvi Wave
