Development and Validation of a Spectrofluorimetric Method for the Estimation of Baicalein in Bulk and Formulation

Dr. Sachin Shivling Bhusari , Aarti Raju Popalghat , Dr.Pravin.S. Wakte

Spectrofluorimetry, Baicalein, Validation,Excitation, Emission

A simple, accurate, precise, sensitive and cost-effective spectrofluorimetric method was developed and validated for the estimation of baicalein in bulk and formulation. The relative fluorescence intensity of baicalein was measured in pure methanol at an excitation wavelength of 371 nm and an emission wavelength 433 nm. Proposed method was found to be linear over the range of 50 to 800 ng/ml with correlation coefficient 0.999. Proposed method was validated using different analytical method validation parameters viz. Accuracy, precision, LOD, LOQ, robustness and ruggedness using QC standards as per the ICH guidelines. The percentage recovery was found to be 101.43% and percentage RSD values were found to be less than 2 for accuracy and precision studies. The detection and quantification limits for the proposed method were found to be 2.14ng/ml and 6.48ng/ml, respectively. A simple, accurate, precise, sensitive yet cost-effective spectrofluorimetric method was developed for the estimation of baicalein in bulk and formulation. The said spectrofluorimetric method was found to be economic as it comprises with methanol: water (40:60) as a solvent.

Published Paper   E-Certificate

"Development and Validation of a Spectrofluorimetric Method for the Estimation of Baicalein in Bulk and Formulation", IJSDR - International Journal of Scientific Development and Research (www.IJSDR.org), ISSN:2455-2631, Vol.8, Issue 6, page no.1910 - 1915, June-2023, Available :https://ijsdr.org/papers/IJSDR2306261.pdf

Volume 8 Issue 6, June-2023

Pages : 1910 - 1915

Paper Reg. ID: IJSDR_207537

Published Paper Id: IJSDR2306261

Downloads: 000347034

Research Area: Pharmacy

Country: Aurangabad, Maharashtra, India

Published Paper PDF: https://ijsdr.org/papers/IJSDR2306261

Published Paper URL: https://ijsdr.org/viewpaperforall?paper=IJSDR2306261

ISSN: 2455-2631 | IMPACT FACTOR: 9.15 Calculated By Google Scholar | ESTD YEAR: 2016

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 9.15 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Publisher: IJSDR(IJ Publication) Janvi Wave

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