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INTERNATIONAL JOURNAL OF SCIENTIFIC DEVELOPMENT AND RESEARCH International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2455-2631 | Impact factor: 8.15 | ESTD Year: 2016 open access , Peer-reviewed, and Refereed Journals, Impact factor 8.15 |
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| Paper Title: | Isolation and screening of cellulose degrading bacteria and optimization of their cellulase activity. |
| Authors Name: | Gayatri Kishor Shete , Sunil R. Jagiasi |
| Unique Id: | IJSDR2306073 |
| Published In: | Volume 8 Issue 6, June-2023 |
| Abstract: | Cellulose, the most abundant organic molecule on Earth and the most abundant constituent of all plant materials, is a linear biopolymer of glucose molecules linked by beta-1,4-glycosidic bonds. Enzymatic hydrolysis of cellulose necessitates the use of synergistic mixtures of hydrolytic enzymes such as endoglucanases, exoglucanases (cellobiohydrolases), and glucosidases. Microorganisms with cellulolytic activity are being considered as potential candidates for biotechnological applications. The study was designed to isolate and screen potent cellulase producing bacteria. For screening purpose congo red agar media was used. Among all isolates, ten isolates showed cellulase activity. On further screening quantitatively by DNSA method, the potent isolate was screened out. Enzyme activity of that isolate was observed as 106mcg/ml/min at 540nm. By optimization Maximum growth of cellulose degradation bacteria was recorded at 37°C and pH 7.0. Among the carbon sources tested, maximum growth was observed in glucose also potassium nitrate was good nitrogen sources for better survival of CDB isolates. And lastly identification of potent isolate was done by 16s rRNA sequence analysis. And it was resulted that the isolate was Brevundimonas diminuta. |
| Keywords: | Cellulase, Exoglucanases Enzymes, Polysaccharides, Optimization. |
| Cite Article: | "Isolation and screening of cellulose degrading bacteria and optimization of their cellulase activity. ", International Journal of Science & Engineering Development Research (www.ijsdr.org), ISSN:2455-2631, Vol.8, Issue 6, page no.489 - 498, June-2023, Available :http://www.ijsdr.org/papers/IJSDR2306073.pdf |
| Downloads: | 000337353 |
| Publication Details: | Published Paper ID: IJSDR2306073 Registration ID:207130 Published In: Volume 8 Issue 6, June-2023 DOI (Digital Object Identifier): Page No: 489 - 498 Publisher: IJSDR | www.ijsdr.org ISSN Number: 2455-2631 |
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