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ISSN Approved Journal No: 2455-2631 | Impact factor: 8.15 | ESTD Year: 2016
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Authors Name: Akshada Laxman Vitnor , Rajeshree Khandre
Unique Id: IJSDR2212086
Published In: Volume 7 Issue 12, December-2022
Abstract: High performance liquid chromatography (HPLC) is an essential analytical tool in assessing drug product. HPLC methods should be able to separate, detect, and quantify the various drugs and drug related degradants that can form on storage or manufacturing, detect and quantify any drugs and drug-related impurities that may be introduced during synthesis. Today HPLC is widely applied for separations and purify cations in a variety of areas including pharmaceuticals, biotechnology, environmental, polymer and food industries. It is accomplished by injection of a small amount of liquid sample into a moving stream of liquid (called the mobile phase) that passes through a column packed with particles of the stationary phase. The separation of a mixture into its components depends on different degrees of retention of each component in the column. HPLC is just one type of liquid chromatography, meaning the mobile phase is a liquid. This review mainly focuses on the HPLC technique its principle, types, instrumentation and applications. Although several analytical techniques are available for the quantification of steroids, their analysis is challenging due to their low levels and complex matrices of the samples. The efficiency and quick separation of the HPLC combined with the sensitivity, selectivity, simplicity, and cost-efficiency of fluorescence, make HPLC coupled to fluorescence detection (HPLC-FLD) an ideal tool for routine measurement and detection of steroids
Keywords: HPLC, Types, Application, Analytical method validation, pharmaceutical analysis
Cite Article: "A REVIEW ON HIGH PERFORMANCE LIQUID CHROMATOGRAPHY", International Journal of Science & Engineering Development Research (www.ijsdr.org), ISSN:2455-2631, Vol.7, Issue 12, page no.556 - 563, December-2022, Available :http://www.ijsdr.org/papers/IJSDR2212086.pdf
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Publication Details: Published Paper ID: IJSDR2212086
Registration ID:203037
Published In: Volume 7 Issue 12, December-2022
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Page No: 556 - 563
Publisher: IJSDR | www.ijsdr.org
ISSN Number: 2455-2631

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