HYDROPHILLIC INTERACTION LIQUID CHROMATOGRAPHY AS A TOOL FOR QUANTIFICATION OF POLAR ANALYTES

Ankita , Amardeep Ankalgi , Kamya Goyal , C.P.S Verma , Mahendra Singh Ashawat

HILIC; Stationary phase; Column; ACN; Water Layer.

: Hydrophilic Interaction liquid chromatography (HILIC) is a preferred seperation method for polar analyte as compare to reversed phase liquid chromatography. The HILIC methods were reported to be cost effetive, accurate , precise and robust especially for polar analytes such as glycans. The stationary phases mostly consist of different functional groups and dependng upon the column chemistry , it is further classified into neutral, charged, and zwitterion phases. HILIC mobile phases composition consist of high concentration of acetonitrile (60-97%) and a low concentration of water ( 3- 40) that make polar analytes to diffuse into surface of stationary phase for the interaction. The review article focued on basic principle, , HILIC stationary phases and its application. Small and large hydrophilic and genuinely polar granules, such as glycans, peptides and proteins, nucleosides, vitamins, phenols, fungicides, venoms, and a wide range of polychrome hydrophilic metabolites are separated using the HILIC system from different pharmaceutical products , food samples , water sample and biological matrices .

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"HYDROPHILLIC INTERACTION LIQUID CHROMATOGRAPHY AS A TOOL FOR QUANTIFICATION OF POLAR ANALYTES ", IJSDR - International Journal of Scientific Development and Research (www.IJSDR.org), ISSN:2455-2631, Vol.7, Issue 9, page no.300 - 304, September-2022, Available :https://ijsdr.org/papers/IJSDR2209047.pdf

Volume 7 Issue 9, September-2022

Pages : 300 - 304

Paper Reg. ID: IJSDR_201611

Published Paper Id: IJSDR2209047

Downloads: 000347255

Research Area: Pharmacy

Country: Kangra, H.P., India

Published Paper PDF: https://ijsdr.org/papers/IJSDR2209047

Published Paper URL: https://ijsdr.org/viewpaperforall?paper=IJSDR2209047

ISSN: 2455-2631 | IMPACT FACTOR: 9.15 Calculated By Google Scholar | ESTD YEAR: 2016

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 9.15 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Publisher: IJSDR(IJ Publication) Janvi Wave

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