Biodegradation of Benzo(a)Pyrene by Bacterial isolates from the Intestinal Gut lining of Bos tarus
URHIBO VOKE OWARIETA
, OMONIGHO ESHAREGOMA SOLOMON , OSHOMA E. CYPRIAN
Bos tarus, gut lining, biodegradation Benzo(a)Pyrene
Crude oil together with its fractionated products is the linchpin of economic development in Nigeria. Since its discovery the country has become quite influential in the global scale but with this feat, activities from both approved and non- approved explorative facilities have endangered the environment as its generated exudates deposited into both the soil-water matrix has evaded many biodegradation stratagem. In spite of the many treatment protocols many toxic compounds including PAHs are left in the environment with members that are deleterious to human health. Benzo(a)Pyrene a four carbon member of the PAH family is arguably one of the most toxic because it has been implicated as a leading cause of various cancers. Bioremediation involving microbial species from different ecological niches have been useful in solving the problems of recalcitrant polyaromatic hydrocarbons from the environment. Polyaromatic hydrocarbons (PAHs) are environmental pollutants that can be found on many surfaces including grasses that are often consumed by ruminants. It was therefore hypothesized that bacteria in the intestine of cow may have the potential to degrade Benzo(a)Pyrene. Bacteria isolated from the intestinal chyme of the large intestine of a healthy cow identified and screened for PAH degradation potential was used for bioremediation of Benzo(a)Pyrene. Two isolates with the highest degradation capacity after preliminary screening tests, was used to inoculate carbon free Bushnell Haas medium containing the PAHs in single and combined cultures for the degradation tests. Samples were withdrawn at intervals of three days and analyzed for bacterial growth and concentration loss of the B(a)P for 16 days. The two test isolates selected after screening and identification were Escherichia coli and Klebsiella pneumoniae. HPLC/GCMS analyses showed that the concentration of Benzo[a]Pyrene declined by 84.8%,91.04% and 96.44% by E. coli, K. pnuemoniae and a combination of both respectively after 16 days. ANOVA confirmed significant differences in the extent of the degradation of the PAHs by the test bacteria and their combined cultures (P<0.05). The growth of the isolates combined peaked at 1.98 log cfu/ml between days 10 and 13 during degradation of B(a)P. Phthalate was the major degradation product in the course of degradation of B(a)P. E. coli and K. pneumoniae were identified by 16S rRNA. It can be concluded that the intestine of Bos tarus harbor strains of bacteria that are capable of a high degree of degradation of B(a)P and E. coli and K. pneumoniae can work synergistically for bioremediation of PAH-polluted environment.
"Biodegradation of Benzo(a)Pyrene by Bacterial isolates from the Intestinal Gut lining of Bos tarus", IJSDR - International Journal of Scientific Development and Research (www.IJSDR.org), ISSN:2455-2631, Vol.7, Issue 6, page no.139 - 152, June-2022, Available :https://ijsdr.org/papers/IJSDR2206023.pdf
Volume 7
Issue 6,
June-2022
Pages : 139 - 152
Paper Reg. ID: IJSDR_200590
Published Paper Id: IJSDR2206023
Downloads: 000347068
Research Area: Life Sciences
Country: EFFURUN, DELTA, Nigeria
ISSN: 2455-2631 | IMPACT FACTOR: 9.15 Calculated By Google Scholar | ESTD YEAR: 2016
An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 9.15 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator
Publisher: IJSDR(IJ Publication) Janvi Wave
