Welcome to IJSDR UGC CARE norms ugc approved journal norms IJRTI Research Journal | ISSN : 2455-2631
IJSDR
INTERNATIONAL JOURNAL OF SCIENTIFIC DEVELOPMENT AND RESEARCH
International Peer Reviewed & Refereed Journals, Open Access Journal
ISSN Approved Journal No: 2455-2631 | Impact factor: 8.15 | ESTD Year: 2016
Scholarly open access journals, Peer-reviewed, and Refereed Journals, Impact factor 8.15 (Calculate by google scholar and Semantic Scholar | AI-Powered Research Tool) , Multidisciplinary, Monthly, Indexing in all major database & Metadata, Citation Generator, Digital Object Identifier(DOI)

Issue: June 2022

Volume 7 | Issue 6

Impact factor: 8.15

Click Here For more Info

Imp Links for Author
Imp Links for Reviewer
Research Area
Subscribe IJSDR
Visitor Counter

Copyright Infringement Claims
Indexing Partner
Published Paper Details
Paper Title: Biodegradation of Benzo(a)Pyrene by Bacterial isolates from the Intestinal Gut lining of Bos tarus
Authors Name: URHIBO VOKE OWARIETA , OMONIGHO ESHAREGOMA SOLOMON , OSHOMA E. CYPRIAN
Unique Id: IJSDR2206023
Published In: Volume 7 Issue 6, June-2022
Abstract: Crude oil together with its fractionated products is the linchpin of economic development in Nigeria. Since its discovery the country has become quite influential in the global scale but with this feat, activities from both approved and non- approved explorative facilities have endangered the environment as its generated exudates deposited into both the soil-water matrix has evaded many biodegradation stratagem. In spite of the many treatment protocols many toxic compounds including PAHs are left in the environment with members that are deleterious to human health. Benzo(a)Pyrene a four carbon member of the PAH family is arguably one of the most toxic because it has been implicated as a leading cause of various cancers. Bioremediation involving microbial species from different ecological niches have been useful in solving the problems of recalcitrant polyaromatic hydrocarbons from the environment. Polyaromatic hydrocarbons (PAHs) are environmental pollutants that can be found on many surfaces including grasses that are often consumed by ruminants. It was therefore hypothesized that bacteria in the intestine of cow may have the potential to degrade Benzo(a)Pyrene. Bacteria isolated from the intestinal chyme of the large intestine of a healthy cow identified and screened for PAH degradation potential was used for bioremediation of Benzo(a)Pyrene. Two isolates with the highest degradation capacity after preliminary screening tests, was used to inoculate carbon free Bushnell Haas medium containing the PAHs in single and combined cultures for the degradation tests. Samples were withdrawn at intervals of three days and analyzed for bacterial growth and concentration loss of the B(a)P for 16 days. The two test isolates selected after screening and identification were Escherichia coli and Klebsiella pneumoniae. HPLC/GCMS analyses showed that the concentration of Benzo[a]Pyrene declined by 84.8%,91.04% and 96.44% by E. coli, K. pnuemoniae and a combination of both respectively after 16 days. ANOVA confirmed significant differences in the extent of the degradation of the PAHs by the test bacteria and their combined cultures (P<0.05). The growth of the isolates combined peaked at 1.98 log cfu/ml between days 10 and 13 during degradation of B(a)P. Phthalate was the major degradation product in the course of degradation of B(a)P. E. coli and K. pneumoniae were identified by 16S rRNA. It can be concluded that the intestine of Bos tarus harbor strains of bacteria that are capable of a high degree of degradation of B(a)P and E. coli and K. pneumoniae can work synergistically for bioremediation of PAH-polluted environment.
Keywords: Bos tarus, gut lining, biodegradation Benzo(a)Pyrene
Cite Article: "Biodegradation of Benzo(a)Pyrene by Bacterial isolates from the Intestinal Gut lining of Bos tarus", International Journal of Science & Engineering Development Research (www.ijsdr.org), ISSN:2455-2631, Vol.7, Issue 6, page no.139 - 152, June-2022, Available :http://www.ijsdr.org/papers/IJSDR2206023.pdf
Downloads: 00096796
Publication Details: Published Paper ID: IJSDR2206023
Registration ID:200590
Published In: Volume 7 Issue 6, June-2022
DOI (Digital Object Identifier):
Page No: 139 - 152
Publisher: IJSDR | www.ijsdr.org
ISSN Number: 2455-2631

Click Here to Download This Article

Article Preview

Click here for Article Preview







Major Indexing from www.ijsdr.org
Google Scholar ResearcherID Thomson Reuters Mendeley : reference manager Academia.edu
arXiv.org : cornell university library Research Gate CiteSeerX DOAJ : Directory of Open Access Journals
DRJI Index Copernicus International Scribd DocStoc

Track Paper
Important Links
Conference Proposal
ISSN
DOI (A digital object identifier)


Providing A digital object identifier by DOI
How to GET DOI and Hard Copy Related
Open Access License Policy
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
Creative Commons License
This material is Open Knowledge
This material is Open Data
This material is Open Content
Social Media
IJSDR

Indexing Partner