INTERNATIONAL JOURNAL OF SCIENTIFIC DEVELOPMENT AND RESEARCH International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2455-2631 | Impact factor: 8.15 | ESTD Year: 2016
open access , Peer-reviewed, and Refereed Journals, Impact factor 8.15
ISOLATION AND CHARACTERIZATION OF CELLULASE PRODUCING BACTERIA FROM SALIVA SAMPLE AND OPTIMIZATION OF EXTRACTED CELLULASE
Authors Name:
Ghanwat Kritika
, Lahudkar Dnyaneshwari ,
Unique Id:
IJSDR2205120
Published In:
Volume 7 Issue 5, June-2022
Abstract:
Microorganisms have a great potential in degradation of cellulose. Among which, bacteria have a major contribution. This study was conducted to isolate and characterize cellulose degrading bacteria which was collected from ‘Jaffrabadi’ breed of buffalo by morphological and biochemical analysis. The cellulose degrading bacteria were isolated, screened and purified on CMC agar plates. Screening resulted in selection of bacterial isolate producing clear zone when plates were flooded with Congo red and washed with NaCl, which confirmed the cellulose degradation. The morphological characterization showed the bacteria to be a Gram positive, motile, nonsporulating rod-shaped bacterium. The biochemical tests indicated that the bacteria belonged to the Cellulomonas spp. (according to Bergey’s Manual of Determinative Bacteriology). The bacterial isolate was then subjected to cellulase production, which was further optimized with various parameters like incubation time, temperature, pH and also with different carbon and nitrogen sources.
"ISOLATION AND CHARACTERIZATION OF CELLULASE PRODUCING BACTERIA FROM SALIVA SAMPLE AND OPTIMIZATION OF EXTRACTED CELLULASE", International Journal of Science & Engineering Development Research (www.ijsdr.org), ISSN:2455-2631, Vol.7, Issue 5, page no.675 - 679, June-2022, Available :http://www.ijsdr.org/papers/IJSDR2205120.pdf
Downloads:
000337212
Publication Details:
Published Paper ID: IJSDR2205120
Registration ID:200481
Published In: Volume 7 Issue 5, June-2022
DOI (Digital Object Identifier):
Page No: 675 - 679
Publisher: IJSDR | www.ijsdr.org
ISSN Number: 2455-2631
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