ISOLATION AND CHARACTERIZATION OF CELLULASE PRODUCING BACTERIA FROM SALIVA SAMPLE AND OPTIMIZATION OF EXTRACTED CELLULASE

Ghanwat Kritika , Lahudkar Dnyaneshwari ,

Jaffrabadi, degrading, characterize, CMC agar, Cellulomonas

Microorganisms have a great potential in degradation of cellulose. Among which, bacteria have a major contribution. This study was conducted to isolate and characterize cellulose degrading bacteria which was collected from ‘Jaffrabadi’ breed of buffalo by morphological and biochemical analysis. The cellulose degrading bacteria were isolated, screened and purified on CMC agar plates. Screening resulted in selection of bacterial isolate producing clear zone when plates were flooded with Congo red and washed with NaCl, which confirmed the cellulose degradation. The morphological characterization showed the bacteria to be a Gram positive, motile, nonsporulating rod-shaped bacterium. The biochemical tests indicated that the bacteria belonged to the Cellulomonas spp. (according to Bergey’s Manual of Determinative Bacteriology). The bacterial isolate was then subjected to cellulase production, which was further optimized with various parameters like incubation time, temperature, pH and also with different carbon and nitrogen sources.

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"ISOLATION AND CHARACTERIZATION OF CELLULASE PRODUCING BACTERIA FROM SALIVA SAMPLE AND OPTIMIZATION OF EXTRACTED CELLULASE", IJSDR - International Journal of Scientific Development and Research (www.IJSDR.org), ISSN:2455-2631, Vol.7, Issue 5, page no.675 - 679, June-2022, Available :https://ijsdr.org/papers/IJSDR2205120.pdf

Volume 7 Issue 5, June-2022

Pages : 675 - 679

Paper Reg. ID: IJSDR_200481

Published Paper Id: IJSDR2205120

Downloads: 000347239

Research Area: Life Sciences 

Country: Dist-Ahmednagar, Maharashtra, India

Published Paper PDF: https://ijsdr.org/papers/IJSDR2205120

Published Paper URL: https://ijsdr.org/viewpaperforall?paper=IJSDR2205120

ISSN: 2455-2631 | IMPACT FACTOR: 9.15 Calculated By Google Scholar | ESTD YEAR: 2016

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 9.15 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Publisher: IJSDR(IJ Publication) Janvi Wave

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