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INTERNATIONAL JOURNAL OF SCIENTIFIC DEVELOPMENT AND RESEARCH International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2455-2631 | Impact factor: 8.15 | ESTD Year: 2016 open access , Peer-reviewed, and Refereed Journals, Impact factor 8.15 |
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| Paper Title: | DETECTION AND ANTIBIOGRAM OF EXTENDED SPECTRUM BETALACTAMASE (ESBL) PRODUCING ESCHERICHIA COLI ISOLATED FROM URINE SAMPLES AT GOVERMENT MEDICAL COLLEGE,KOTA RAJASTHAN |
| Authors Name: | Dr. Bhupendra Kumar Mandawat |
| Unique Id: | IJSDR1907047 |
| Published In: | Volume 4 Issue 7, July-2019 |
| Abstract: | ABSTRACT: The spread of Extended Spectrum Beta-Lactamases (ESBLs) producing bacteria has become strikingly rapid worldwide, indicating that continuous monitoring systems and effective infection control measures are absolutely required. The therapeutic options for the infections which are caused by these organisms have also become increasingly limited. ESBLs producing strains have emerged as a significant challenge to counter with present antibiotics. BACKGROUND: Escherichia coli is the most common organism causing urinary tract infection (UTI). This organism has the ability to produce ESBLs which confer multiple drug resistance making urinary tract infection difficult to treat. OBJECTIVES: The aims of this study were to detect prevalence of ESBL production among E. coli isolates in urine samples and to detect their antibiotic susceptibility pattern at Govt. Medical College, Kota. This study demonstrate the importance of regular review of empirical antibiotic therapy for UTI in view of the evolving resistance of ESBL producing E. coli to commonly used antimicrobial agents. METHODOLOGY: The present study was conducted in the clinical microbiology laboratory of Govt. Medical College, Kota from period of 1st January, 2018 to 31th December 2018 to evaluate prevalence of ESBL production among E. coli isolates in urine samples and to detect their antibiotic susceptibility pattern. RESULT: A total of 500 consecutive, nonrepetitive E. coli isolates were studied. Antimicrobial susceptibility test was performed on Muller Hinton agar plates using Kirby Bauer disk diffusion method. ESBL detection was done for all isolates according to latest CLSI criteria. These isolates were confirmed for ESBL production by the double disc synergy test (DDST) and the phenotypic confirmatory disc diffusion test (PCDDT) and Twenty-five randomly selected isolates were further confirmed by the E-test ESBL strip randomly. Out of 500 E. coli isolate, 310(62%) were ESBL producers and 190(38%) were Non ESBL producers. Out of the 310 isolates which were tested,228(73.55%) were found to be ESBL producers by PCDDT,193 (62.26%) were found to be ESBL producers by DDST and 19(76%), were found to be ESBL producers by the E-test ESBL strip which showed a highly significant correlation with PCDDT. Conclusion: As results showed that there was a high prevalence of ESBL production in our setup so, it is essential to report the ESBL production along with the routine sensitivity reports, which will help the clinician in selection of proper antibiotics. |
| Keywords: | ESBLs, UTI, ESCHERICHIA COLI, CLSI, DDST, PCDDT, E-test ESBL STRIP |
| Cite Article: | "DETECTION AND ANTIBIOGRAM OF EXTENDED SPECTRUM BETALACTAMASE (ESBL) PRODUCING ESCHERICHIA COLI ISOLATED FROM URINE SAMPLES AT GOVERMENT MEDICAL COLLEGE,KOTA RAJASTHAN", International Journal of Science & Engineering Development Research (www.ijsdr.org), ISSN:2455-2631, Vol.4, Issue 7, page no.272 - 279, July-2019, Available :http://www.ijsdr.org/papers/IJSDR1907047.pdf |
| Downloads: | 000337349 |
| Publication Details: | Published Paper ID: IJSDR1907047 Registration ID:190798 Published In: Volume 4 Issue 7, July-2019 DOI (Digital Object Identifier): Page No: 272 - 279 Publisher: IJSDR | www.ijsdr.org ISSN Number: 2455-2631 |
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