Genetic diversity and phylogenetic relationship among spineless cactus species accessions (opuntia ficus-indica) as revealed by ISSR markers

J. Serena Jasmine , A. R. Aher , V. P. Chimote , P. L. Kulwal , L. N. Tagad

Opuntia ficus-indica, Genetic diversity, ISSR markers, Phylogenetic relationship, Spineless cactus.

The present study, “Genetic diversity and phylogenetic relationship among spineless cactus species accession (Opuntia ficus-indica) as revealed by ISSR markers”, characterized genetic variability and relatedness among diverse spineless cactus genotypes from Italy, Brazil, Mexico, the USA and Chile. Genomic DNA from cladode tissue was amplified using ISSR primers, producing clear and highly polymorphic banding patterns. Substantial variation was observed, with both shared and rare alleles detected; accession 1281 demonstrated distinctiveness through the presence of unique alleles. Cluster analysis grouped most accessions by geographical origin and breeding background, while 1281 formed a separate divergent cluster. Brazilian forage types (IPA, COPENA series) exhibited strong similarity, whereas Italian and Mexican accessions showed notable divergence. The results confirm ISSR markers as effective tools for dissecting genetic variability in cactus germplasm, highlighting redundancy in some breeding lines and the presence of unique genetic resources. These findings provide a molecular framework for conservation and breeding, underscoring the importance of rare alleles in enhancing stress tolerance, fruit quality and adaptability in arid agriculture.

Published Paper   E-Certificate

"Genetic diversity and phylogenetic relationship among spineless cactus species accessions (opuntia ficus-indica) as revealed by ISSR markers", IJSDR - International Journal of Scientific Development and Research (www.IJSDR.org), ISSN:2455-2631, Vol.10, Issue 9, page no.a788-a801, September-2025, Available :https://ijsdr.org/papers/IJSDR2509094.pdf

Volume 10 Issue 9, September-2025

Pages : a788-a801

Paper Reg. ID: IJSDR_304892

Published Paper Id: IJSDR2509094

Downloads: 00070

Research Area: Biological Science

Country: Coimbatore, Tamil Nadu, India

Published Paper PDF: https://ijsdr.org/papers/IJSDR2509094

Published Paper URL: https://ijsdr.org/viewpaperforall?paper=IJSDR2509094

ISSN: 2455-2631 | IMPACT FACTOR: 9.15 Calculated By Google Scholar | ESTD YEAR: 2016

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 9.15 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Publisher: IJSDR(IJ Publication) Janvi Wave

This work is licensed under a Creative Commons Attribution 4.0 International License and The Open Definition