Metagenomic Exploration of Microbial Diversity of Industrial Effluent Contaminated Soils of Silvassa, India.

Bariya Nipun , Dr. Mayuri Dholaria , Dr. Aparna Jitesh Tailor

COG, KEGG, Metagenomes, Microbial Diversity, Next generation sequencing.

This study reports a metagenomic approach, to investigate the microbial diversity of soils contaminated with different industrial effluents. Three different contaminated soil samples were collected from different industrial sites or Rakholi, Surangi and Kherdi, in Silvassa region. Total soil DNA was extracted and whole genome sequencing Library was prepared and amplified using universal primers. The genomic library was quantified and validated by Qubit and 4150 Tapestation system, followed by sequencing and bioinformatic analysis of metagenome, to identify microbial diversity in the contaminated soil. Microbial diversity analysis represented the dominance of Pseudomonadota, Actinomycetota, Bacillota, Myxococcota, Acidobacteriota and Thermodesulfobacteriota in all the three metagenomes. Presence of pollutant degrading microbes such as Bacillus, Clostridium, Candidatus Entotheonella, Pseudomonas and Desulforomonas strongly indicates their bioremediation ability. KEGG and COG annotated main functional categories included carbohydrate metabolism, amino acids metabolism, and protein metabolism. This study explored the still unexplored soil bacterial diversity, through metagenomic approach, and is the first ever report for the region of Silvassa, Dadra & Nagar Haveli, India. Further deep investigation shall reveal novel species or proteins produced by microbial community, with potential applications towards sustainable environment.

Published Paper   E-Certificate

"Metagenomic Exploration of Microbial Diversity of Industrial Effluent Contaminated Soils of Silvassa, India.", IJSDR - International Journal of Scientific Development and Research (www.IJSDR.org), ISSN:2455-2631, Vol.9, Issue 6, page no.1232 - 1250, June-2024, Available :https://ijsdr.org/papers/IJSDR2406145.pdf

Volume 9 Issue 6, June-2024

Pages : 1232 - 1250

Paper Reg. ID: IJSDR_211887

Published Paper Id: IJSDR2406145

Downloads: 000347091

Research Area: Life Sciences 

Country: SILVASSA, DADRA AND NAGAR HAVELI, India

Published Paper PDF: https://ijsdr.org/papers/IJSDR2406145

Published Paper URL: https://ijsdr.org/viewpaperforall?paper=IJSDR2406145

DOI: https://doi.org/10.5281/zenodo.12596888

ISSN: 2455-2631 | IMPACT FACTOR: 9.15 Calculated By Google Scholar | ESTD YEAR: 2016

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 9.15 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Publisher: IJSDR(IJ Publication) Janvi Wave

This work is licensed under a Creative Commons Attribution 4.0 International License and The Open Definition