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INTERNATIONAL JOURNAL OF SCIENTIFIC DEVELOPMENT AND RESEARCH International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2455-2631 | Impact factor: 8.15 | ESTD Year: 2016 open access , Peer-reviewed, and Refereed Journals, Impact factor 8.15 |
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| Paper Title: | Ultra-Performance Supercritical Fluid Chromatography Hyphenated to Tandem Mass Spectrometry for a Novel Targeted Analysis of Peripheral Steroids |
| Authors Name: | Kondepudi Lalitha , Sanikommu Kalpana , Saripalli Sri Lakshmi |
| Unique Id: | IJSDR2305191 |
| Published In: | Volume 8 Issue 5, May-2023 |
| Abstract: | An alternate technique for steroid analysis is ultra-performance supercritical fluid chromatography-tandem mass spectrometry (UPSFC-MS/MS). In order to deliver relevant and thorough results, it is crucial that analytical procedures for steroid profiling are continuously improved. This study's objective was to rapidly and accurately detect and measure a large number of endogenous steroids from the four main classes (oestrogens, androgens, progestogens, and corticosteroids). This brand-new UPSFC-MS/MS technique uses electrospray in positive ionization (ESI+) mode, which is robust, selective, and sensitive enough to profile 19 steroid compounds in 50 litres of human plasma. In the multiple reaction monitoring (MRM) mode, 19 distinct steroids were successfully isolated under optimal conditions. With correlation coefficients (R2) in the range of 0.9883 to 0.9999 and a calibration range of 0.05 to 500 ng/mL in human plasma, the method's linearity was good. The method's intraday and interday precision, measured as RSD, was under 15%. The nineteen analytes' accuracy ranged from 80 to 116%. Finally, the innovative technology was successfully used to identify nineteen steroids in under five minutes, opening the door for their potential usage in both ordinary medical treatment and research. |
| Keywords: | UPSFC-MS/MS, ESI+, MRM, APCI, APPI, BEH, CSH Fluorophenyl |
| Cite Article: | "Ultra-Performance Supercritical Fluid Chromatography Hyphenated to Tandem Mass Spectrometry for a Novel Targeted Analysis of Peripheral Steroids", International Journal of Science & Engineering Development Research (www.ijsdr.org), ISSN:2455-2631, Vol.8, Issue 5, page no.1205 - 1209, May-2023, Available :http://www.ijsdr.org/papers/IJSDR2305191.pdf |
| Downloads: | 000337070 |
| Publication Details: | Published Paper ID: IJSDR2305191 Registration ID:206319 Published In: Volume 8 Issue 5, May-2023 DOI (Digital Object Identifier): Page No: 1205 - 1209 Publisher: IJSDR | www.ijsdr.org ISSN Number: 2455-2631 |
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