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| Paper Title: | COMPARATIVE ANALYSIS OF ANIFUNGAL ACTIVITY OF FOUR ESSENTIAL OILS AGAINST DIFFERENT FUNGI |
| Authors Name: | Geethanjali R , Aathiya Vanekar , Pratibha K.Y , Marhoob Banu |
| Unique Id: | IJSDR2305069 |
| Published In: | Volume 8 Issue 5, May-2023 |
| Abstract: | The present study was conducted with the main purpose to compare the antifungal activity of four essential oils viz., Clove oil (Syzygium aromaticum), Eucalyptus oil (Eucalyptus globulus), Neem oil (Azadirachta indica) and Rose oil (Rosa rubiginosa) at concentrations of 20%, 40%, 60%, 80% and 100% respectively on Aspergillus fumigatus, Aspergillus niger and Fusarium oxysporum associated with Sorghum vulgare and Zea mays by poisoned food technique. Screening of fungi on Sorghum vulgare and Zea mays seeds was done by agar plate method and the fungi studied were Aspergillus fumigatus, Aspergillus niger and Fusarium oxysporum. Potato dextrose agar was prepared and well grown colonies of fungi were inoculated. Pure cultures of fungi were obtained by subculturing the previous culture and incubated at 27 ± 2 °C for 48 hours. Fungus was suspended in sterile distilled water and adjusted to a standard inoculum size to 1-2 x 106 CFU/mL. Five different concentrations of essential oils were added to sterile potato dextrose agar at concentration of 500 ppm in the hood of laminar air flow and 0.1 ml of fungal suspension was used to inoculate treated PDA petriplates with a sterile non-toxic cotton swab. Acetone at 500 ppm was added as control. The treated plates with essential oils and control were incubated at 27 ± 2 °C for 7 days. After incubation the treated plates were observed for zone of inhibition around wells and also for complete inhibition. Zone of inhibition were measured and recorded in millimetres. |
| Keywords: | Agar plate method, Antifungal activity, Poisoned food technique, Sorghum vulgare, Zea mays. |
| Cite Article: | "COMPARATIVE ANALYSIS OF ANIFUNGAL ACTIVITY OF FOUR ESSENTIAL OILS AGAINST DIFFERENT FUNGI", International Journal of Science & Engineering Development Research (www.ijsdr.org), ISSN:2455-2631, Vol.8, Issue 5, page no.506 - 510, May-2023, Available :http://www.ijsdr.org/papers/IJSDR2305069.pdf |
| Downloads: | 000223171 |
| Publication Details: | Published Paper ID: IJSDR2305069 Registration ID:206056 Published In: Volume 8 Issue 5, May-2023 DOI (Digital Object Identifier): Page No: 506 - 510 Publisher: IJSDR | www.ijsdr.org ISSN Number: 2455-2631 |
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