INTERNATIONAL JOURNAL OF SCIENTIFIC DEVELOPMENT AND RESEARCH International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2455-2631 | Impact factor: 8.15 | ESTD Year: 2016
open access , Peer-reviewed, and Refereed Journals, Impact factor 8.15
Two Black gram varieties LBG-806 (resistant variety) and LBG-823 (sensitive variety) were selected for comparative analysis of molecular responses at different temperature treatments (as per TIR protocol). Temperature Induction treatment promote the plant hardening by acquiring thermo-tolerance through several mechanisms. Both the selected cultivars showed varied banding pattern with ISSR-PCR amplification at different treatments. Results of ISSR-PCR amplification of DNA showed higher number of bands in induction treatment than non-induction treatment both in resistant and susceptible varieties. Two extra bands were observed in resistant variety upon induction treatment, one band at 600-700bp for UBC-807 primer and another band at 700bp for UBC-853 primer. Presence of high intensity bands and extra bands in both varieties in induction treatment indicated that they have been acclimated and hardened at the time of induction temperature, as a result protected even at the time of lethal temperature. It is concluded that marker assisted analysis between resistant and susceptible varieties with different treatments as per TIR protocol provided supportive molecular information for relevance of TIR based screening technique.
Keywords:
TIR, Black gram, SDS-PAGE, ISSR-PCR
Cite Article:
"A Comparative Analysis of Genetic Variability for Acquired Thermo-tolerance in Two Black gram Varieties using ISSR markers", International Journal of Science & Engineering Development Research (www.ijsdr.org), ISSN:2455-2631, Vol.8, Issue 2, page no.530 - 536, February-2023, Available :http://www.ijsdr.org/papers/IJSDR2302093.pdf
Downloads:
000336256
Publication Details:
Published Paper ID: IJSDR2302093
Registration ID:203943
Published In: Volume 8 Issue 2, February-2023
DOI (Digital Object Identifier): https://doi.org/10.5281/zenodo.10101900
Page No: 530 - 536
Publisher: IJSDR | www.ijsdr.org
ISSN Number: 2455-2631
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