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INTERNATIONAL JOURNAL OF SCIENTIFIC DEVELOPMENT AND RESEARCH International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2455-2631 | Impact factor: 8.15 | ESTD Year: 2016 open access , Peer-reviewed, and Refereed Journals, Impact factor 8.15 |
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| Paper Title: | ISOLATION AND CHARACTERISATION OF INDIAN LIQUORICE SEEDS BY UV-VIS SPECTROSCOPY, FT-IR, RP-HPLC, AND GC-MS |
| Authors Name: | S.Dharmarajsanthosam , P.Selvam , Abhinandan Danodia |
| Unique Id: | IJSDR2302091 |
| Published In: | Volume 8 Issue 2, February-2023 |
| Abstract: | Abstract Abrus precatorius plant is commonly called Indian liquorice, Rosary pea, Jequirity bean, crab'eye, and Gunja. The methanol crude extract CMME was prepared from the dried powdered Abrus precatorius seeds by the maceration method for 7 days. The compounds from the crude extract CMME were isolated by column chromatography using silica gel 60-120 mesh as the adsorbent. The isolated compounds are further purified by Thin Layer Chromatography. The compound that has the same RF value is combined. A total of three compounds are isolated from CMME and are named CMME I, CMME II, and CMME III. The first-eluted, second-eluted, and third-eluted compounds are CMME I, CMME II, and CMME III respectively. The RF value of the isolated compounds was determined by using a small TLC chamber and methanol as solvent. The spot was determined by the Iodine chamber method. The RF values of CMME I, CMME II, and CMME III were found to be 0.92, 0.74, and 0.67 respectively. The isolated compounds were characterized by UV-VIS spectroscopy, FT-IR, RP-HPLC, and GC-MS. The λmax of the crude extract CMME was found to be 346.20nm. The λmax of the separated compounds CMME I, CMME II, and CMME III was found to be 355.45nm, 318.17nm, and 298.20nm correspondingly by using solvent methanol as blank. The absorption frequencies from 4000-667 cm-1 were used for the structure elucidation, (4000 cm-1-1300 cm-1) was used as the high-frequency region and (1300 cm-1 – 667 cm-1) was used as the fingerprint region for the interpretation of CMME I, CMME II and CMME III by FTIR spectroscopy. In the RP-HPLC chromatogram 1.226min, 2.038min and 8.963 min is the retention time for three isolates CMME I, CMME II & CMME III correspondingly. The peak present at the m/z ratio 281.0 of the GC-MS spectra of compound CMME I indicate the presence of (4, 6-O- Benzylidene) methyl-alpha D-glucopyranoside, molecular formula C14H18O6.The peak present at the m/z ratio 222 and 429.1 of the GC-MS spectra of compound II indicates the presence of isoflavone base and the presence of Malonyl Hexose respectively. So the presence of the peaks at the m/z of 222 and 429.1 of the GC-MS spectra of compound CMME II indicate the presence of Isoflavone base 20-O-malonyl Hex. The peak present at the m/z ratio 175.0 of the GC-MS spectra of compound CMME III indicates the presence of Trigonelline hydrochloride, molecular formula C7H8ClNO2.The retention time of the CMME I, CMME II, and CMME III was found to be 16.969, 17.253, and 17.310 min respectively. |
| Keywords: | Keywords: UV-VIS spectroscopy, FT-IR, RP-HPLC, GC-MS. |
| Cite Article: | "ISOLATION AND CHARACTERISATION OF INDIAN LIQUORICE SEEDS BY UV-VIS SPECTROSCOPY, FT-IR, RP-HPLC, AND GC-MS", International Journal of Science & Engineering Development Research (www.ijsdr.org), ISSN:2455-2631, Vol.8, Issue 2, page no.519 - 525, February-2023, Available :http://www.ijsdr.org/papers/IJSDR2302091.pdf |
| Downloads: | 000337070 |
| Publication Details: | Published Paper ID: IJSDR2302091 Registration ID:204013 Published In: Volume 8 Issue 2, February-2023 DOI (Digital Object Identifier): Page No: 519 - 525 Publisher: IJSDR | www.ijsdr.org ISSN Number: 2455-2631 |
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