Isolation, Identification, Antimicrobial screening of micro-organism from the natural source.
Neha Umesh Vadagaonkar
, Aishwarya Shahaji Tambekar , Trupti Appasaheb Todkar , Vikas Kantilal Tupe , Divya Madhukar Unale
Antimicrobial, Micro-organism, Water Sources
In natural ecosystems, microorganisms play a highly specialised role in the recycling of resources and water purification. Bacteria and fungi predominate in aquatic systems. lactobacilli have recently been found to be useful in combating various pathogens, limited data exists on their therapeutic potential for saureas infection. The increasing prevalrnce of methicillin-resistant staphylococcus aureus has become a major threat to public health. Lactic acid fermentation is a natural method of antimicrobial food protection. Antagonistic activity of Lactobacillus sp. bacteria,taking part in this process, is directed mainly against the same or other microorganisms. In this work we determine the impactof the presence of xylitol and galactosyl-xylitol on the antagonistic activity of 60 Lactobacillus sp. strains against indicator molds(Alternaria alternata, Alternaria brassicicola, Aspergillus niger, Fusarium latenicum, Geotrichum candidum, and Mucor hiemalis)and yeasts (Candida vini). We used double-layer method to select antifungal strains of Lactobacillus bacteria and poisoned mediummethod to confirm their fungistatic properties. Additionally, we examined the inhibition of Alternaria brassicicola by Lactobacillusparacasei ŁOCK 0921 cultivated with xylitol or galactosyl-xylitol directly on wild cherries. The presence of xylitol and its galactosylderivative led to increase of spectrum of antifungal activity in most of the studied plant-associated lactobacilli strains. However,no single strain exhibited activity against all the indicator microorganisms. The antifungal activity of Lactobacillus bacteria againstmolds varied considerably and depended on both the indicator strain and the composition of the medium. The presence of xylitoland galactosyl-xylitol in the growth medium is correlated with the antifungal activity of the studied Lactobacillussp. bacteria againstselected indicator molds. Lactic acid bacteria (LAB) are known to be capable of inhibiting pathogenic and degrading microorganisms, bringing desirable changes in taste and texture leading to different natural antimicrobials production. These features have encouraged the search for new strains with technological potential (Tulini et al., 2016). On the other hand, LAB give flavor and preserve foods by producing antimicrobial substances such as lactic and acetic acids, hydrogen peroxide, diacetyl, carbon dioxide, ethanol, bacitracin, reuterin and reutericyclin (Aymerich et al., 2000; Messens et al., 2002; Gálvez et al., 2007). Bacteriocins and other metabolites as LAB productions are regarded generally as safe compounds. The other advantage of LAB is their non-toxic effects (Carr et al., 2002; Cotter et al., 2005). A total of 56 LAB were isolated by Jabbari et al. (2017) and 12 of them were identified by using biochemical methods and 11 were identified using molecular method. Antimicrobial activity tests were performed using disc diffusion method and Staph. aureus ATCC 25923 exhibited 15 ± 0.3 mm antimicrobial activity. Macaluso et al. (2016) obtained 699 LAB strains isolated from traditional Sicilian cheese and raw milk. L. monocytogenes ATCC 7644, Staph. aureus, E. coli and S. Enteritidis bacteria were used as indicators for antimicrobial activity. A total of 223 strains were found to inhibit L. monocytogenes growth. It has been reported that adding bacteriocin-producing cultures is a practical and cost-effective method to improve product quality and safety. The main cause of antimicrobial resistance is the inappropriate and excessive use of antibiotics in humans and animals.
"Isolation, Identification, Antimicrobial screening of micro-organism from the natural source. ", IJSDR - International Journal of Scientific Development and Research (www.IJSDR.org), ISSN:2455-2631, Vol.8, Issue 1, page no.30 - 33, January-2023, Available :https://ijsdr.org/papers/IJSDR2301007.pdf
Volume 8
Issue 1,
January-2023
Pages : 30 - 33
Paper Reg. ID: IJSDR_203320
Published Paper Id: IJSDR2301007
Downloads: 000347234
Research Area: Pharmacy
Country: Kolhapur, Maharashtra, India
ISSN: 2455-2631 | IMPACT FACTOR: 9.15 Calculated By Google Scholar | ESTD YEAR: 2016
An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 9.15 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator
Publisher: IJSDR(IJ Publication) Janvi Wave
