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INTERNATIONAL JOURNAL OF SCIENTIFIC DEVELOPMENT AND RESEARCH International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2455-2631 | Impact factor: 8.15 | ESTD Year: 2016 open access , Peer-reviewed, and Refereed Journals, Impact factor 8.15 |
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| Paper Title: | Comparison of different staining method for identification of Helicobacter pylori in Upper Gastro-intestinal biopsies |
| Authors Name: | DR. NAVDEEP KAUR , Dr Reeta Dhar , Dr Arpita Singh , Dr Shilpi Sahu |
| Unique Id: | IJSDR2210079 |
| Published In: | Volume 7 Issue 10, October-2022 |
| Abstract: | Helicobacter Pylori is a gram negative, motile microorganism that is one of the major causes of chronic gastritis. Helicobacter pylori can be detected by routine hematoxylin and eosin (H&E) stain but the detection should be supported by special stains like Giemsa stain, Warthin-Starry stain, and Modified Methylene blue and Alcian blue. Aim: This study aims to compare the results in detecting H. pylori obtained on special stains like Giemsa stain and Warthin-Starry stain along with H&E stain. Materials and Methods: A prospective study was conducted among 50 upper GI tract endoscopic biopsies obtained from patients presenting with upper GI symptoms like dyspepsia, epigastric pain, hyperacidity at Surgery OPD in a tertiary care hospital. The sensitivity, specificity, PPV and NPV of H&E, modified Giemsa stain and Warthin-Starry stain were calculated for detection of H. pylori and comparisons were drawn. Results: Out of the 50 cases, 27 (54%) tested positive for H. pylori and 23 (46%) were negative. Chronic gastritis was the most common histopathological diagnosis with 15 (56%) cases being H. pylori positive. Warthin-starry stain showed the highest sensitivity, specificity, PPV and NPV out of all three stains. Conclusion: This study highlighted that although routine H&E and Giemsa staining is simple, rapid and cost-effective method in comparison to Warthin starry stain which is costlier and time consuming yet proved to be the gold standard for diagnosis of H. pylori due to its high sensitivity, specificity, PPV and NPV and good colour contrast. |
| Keywords: | H. pylori, Modified Giemsa, Warthin-Starry, sensitivity, specificity |
| Cite Article: | "Comparison of different staining method for identification of Helicobacter pylori in Upper Gastro-intestinal biopsies", International Journal of Science & Engineering Development Research (www.ijsdr.org), ISSN:2455-2631, Vol.7, Issue 10, page no.455 - 461, October-2022, Available :http://www.ijsdr.org/papers/IJSDR2210079.pdf |
| Downloads: | 000337070 |
| Publication Details: | Published Paper ID: IJSDR2210079 Registration ID:202112 Published In: Volume 7 Issue 10, October-2022 DOI (Digital Object Identifier): Page No: 455 - 461 Publisher: IJSDR | www.ijsdr.org ISSN Number: 2455-2631 |
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