INTERNATIONAL JOURNAL OF SCIENTIFIC DEVELOPMENT AND RESEARCH International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2455-2631 | Impact factor: 8.15 | ESTD Year: 2016
open access , Peer-reviewed, and Refereed Journals, Impact factor 8.15
Prevalence of Extended-Spectrum Beta Lactamase producing gram negative bacteria from vaginal infection
Authors Name:
SWATHI M
, Dr. P. SUGANTHI , Dr. P. Sankarganesh , Dr .A. Ganesh Kumar , K. Vasuki
Unique Id:
IJSDR2209130
Published In:
Volume 7 Issue 9, September-2022
Abstract:
Abstract: ESBL producing gram-ve microbial outbreak in bacterial vaginosis is more critical especially during pregnancy. The purpose of this study is to determine the isolate and identify the ESBL antibiotic sensitivity patterns and resistance patterns of the isolates identified from vagionosis cases. A total of 200 high vaginal swabs (HVS) were obtained from pregnant (155 no) and non-pregnant (45 no) womens. The highly positive isolate was further subjected to antibiotic sensitivity tests, and minimal inhibitory concentration tests and Extended-spectrum -lactamase (ESBL) determination tests such as the combined disc method and double disc synergy test were used to investigate resistance patterns, followed by genotypic analysis to knockout resistance genes. Ambler class A beta lactamase genes such as TEM (404 bp), SHV (294 bp), and CTX-M (415 bp) were used as standards. Out of 200 HVS samples, 91 samples positive for Escherichia coli (41nos). Among the 45 isolates, 41 isolates were highly resistant to ESBL antibiotics screened by combined disc diffusion and E-strip test. During the Minimal Inhibitory Concentration analysis, at least 36 had MIC50 of 2 µg/ml, MIC90 of 512 µg/ml and 5 were intermediate to Cefotaxime antibiotics. 50% of isolates obtained from pregnancy samples showed ESBL production. The prevalence of drug resistance genes such as TEM, SHV, CTX-M also confirmed. The current study concluded that pregnant women between the ages of 20 - 30 had a higher rate risk for ESBL producers than non-pregnants womens.
Keywords:
ESBL, CTX-M, Pregnancy,AST
Cite Article:
"Prevalence of Extended-Spectrum Beta Lactamase producing gram negative bacteria from vaginal infection", International Journal of Science & Engineering Development Research (www.ijsdr.org), ISSN:2455-2631, Vol.7, Issue 9, page no.819 - 824, September-2022, Available :http://www.ijsdr.org/papers/IJSDR2209130.pdf
Downloads:
000336256
Publication Details:
Published Paper ID: IJSDR2209130
Registration ID:201849
Published In: Volume 7 Issue 9, September-2022
DOI (Digital Object Identifier):
Page No: 819 - 824
Publisher: IJSDR | www.ijsdr.org
ISSN Number: 2455-2631
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