INTERNATIONAL JOURNAL OF SCIENTIFIC DEVELOPMENT AND RESEARCH International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2455-2631 | Impact factor: 8.15 | ESTD Year: 2016
open access , Peer-reviewed, and Refereed Journals, Impact factor 8.15
DETECTION OF ACE(COLLAGEN BINDING PROTEIN) GENE FROM ENTEROCOCCUS FAECALIS IN PATIENTS WITH ENDODONTIC INFECTIONS
Authors Name:
Keerthana Balaji
, Dr. Dinesh Prabu
Unique Id:
IJSDR2104092
Published In:
Volume 6 Issue 4, April-2021
Abstract:
ABSTRACT: AIM AND OBJECTIVE: To detect ace (collagen binding protein) gene from Enterococcus faecalis from patients with endodontic infections. BACKGROUND: The ace gene helps in the production of angiotensin converting enzyme which plays a major role in the regulation of blood pressure.Adherence of Enterococcus faecalis isolates to extracellular proteins such as collagen I and IV and laminin; identified an E.faecalis-specific gene,ace,whose encoded protein has characteristics of bacterial adhesion and implicated ace in binding to collagen type-I since Enterococcus faecalis has long been implicated in persistent root canal infections and endodontic infections. MATERIALS AND METHODS: A total of 20 samples were collected from patients undergoing endodontic treatment. The isolates were subjected to conventional tests and confirmed, DNA was extracted, quantified and identified sphectrophotometrically and electrophoretically.The ace ( collagen binding protein) was amplified by PCR amplification technique, using specific primer and conditions. RESULT: Out of 20 isolates 2 isolates were shown to express ace (collagen binding protein) gene.
Keywords:
E.faecalis , ace gene , endodontic infections.
Cite Article:
"DETECTION OF ACE(COLLAGEN BINDING PROTEIN) GENE FROM ENTEROCOCCUS FAECALIS IN PATIENTS WITH ENDODONTIC INFECTIONS ", International Journal of Science & Engineering Development Research (www.ijsdr.org), ISSN:2455-2631, Vol.6, Issue 4, page no.560 - 562, April-2021, Available :http://www.ijsdr.org/papers/IJSDR2104092.pdf
Downloads:
000337071
Publication Details:
Published Paper ID: IJSDR2104092
Registration ID:193217
Published In: Volume 6 Issue 4, April-2021
DOI (Digital Object Identifier):
Page No: 560 - 562
Publisher: IJSDR | www.ijsdr.org
ISSN Number: 2455-2631
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