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INTERNATIONAL JOURNAL OF SCIENTIFIC DEVELOPMENT AND RESEARCH International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2455-2631 | Impact factor: 8.15 | ESTD Year: 2016 open access , Peer-reviewed, and Refereed Journals, Impact factor 8.15 |
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| Paper Title: | Assessment of the prevalence of periodontal pathogens in the plaque and gingiva of patients with fixed and removable orthodontic appliance |
| Authors Name: | Kadambari Sriram , Dr. Sri Rengalakshmi.M |
| Unique Id: | IJSDR2103002 |
| Published In: | Volume 6 Issue 3, March-2021 |
| Abstract: | AIM: To assess the prevalence of periodontal pathogens in patients with fixed and removable orthodontic appliance. INTRODUCTION: Fixed and removable orthodontic appliances may complicate an optimal oral hygiene, and this may result in accumulation of dental plaque and gingival inflammation. Evidence indicates the gram-negative obligate anaerobe Porphyromonas gingivalis and A.actinomycetecomitans as putative periodontal pathogens in subgingival dental plaque. P. gingivalis plays an important role in the onset and progression of periodontal diseases, and it is implicated as an indicator of periodontal disease. The aim of this study was to evaluate the occurrence of periodontal pathogens in patients with clinical manifestation of plaque-associated gingivitis treated with fixed and removable orthodontic appliances. MATERIALS AND METHODS: The study was done using convenience sampling of 10 patients undergoing orthodontic treatment from the Orthodontics Department of Saveetha Dental College, Chennai, out of whom 5 were undergoing fixed orthodontic treatment and 5 were wearing removable orthodontic appliances. The study compared the growth of two periodontal pathogens, Porphyromonas gingivalis and Actinobacillus actinomycetecomitans in the plaque deposits collected during the orthodontic treatment. Subgingival plaque samples were collected by inserting a sterile dental curette into the bottom of the gingival crevice during the clinical examination. The samples were collected from the labial-medial and labial-distal surfaces of teeth 31, 32, 41, and 42. The samples from each tooth were pooled into an Eppendorf tube containing 1 mL of sterile saline and stored immediately at -70 degree Centigrade. Analysis was carried out by Real-time PCR. RESULT: Quantification of organisms, Porphyromonas gingivalis and Actinobacillus actinomycetecomitants, have been done for the samples F1,F2,F3,F4,F5 and RA1, RA2, RA3, RA4, RA5 by quantitative PCR (qPCR). It has been found that sample F2 and RA4 has a maximum count of 4178.61943 and 565.56 per ml respectively of P.gingivalis. It has also been found that sample F2 and RA5 has a maximum count of 3821.7132 and 231.59 per ml of A.actinomycetecomitans respectively. CONCLUSION: To create awareness about the occurrence of plaque-associated gingivitis due to periodontal pathogens in patients treated with fixed and removable appliances. |
| Keywords: | Fixed orthodontic treatment, Removable appliance, Periodontal examination, Porphyromonas gingivalis, Actinobacillus actinomycetecomitans, Real-time PCR |
| Cite Article: | "Assessment of the prevalence of periodontal pathogens in the plaque and gingiva of patients with fixed and removable orthodontic appliance", International Journal of Science & Engineering Development Research (www.ijsdr.org), ISSN:2455-2631, Vol.6, Issue 3, page no.7 - 15, March-2021, Available :http://www.ijsdr.org/papers/IJSDR2103002.pdf |
| Downloads: | 000337074 |
| Publication Details: | Published Paper ID: IJSDR2103002 Registration ID:192955 Published In: Volume 6 Issue 3, March-2021 DOI (Digital Object Identifier): Page No: 7 - 15 Publisher: IJSDR | www.ijsdr.org ISSN Number: 2455-2631 |
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