INTERNATIONAL JOURNAL OF SCIENTIFIC DEVELOPMENT AND RESEARCH International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2455-2631 | Impact factor: 8.15 | ESTD Year: 2016
open access , Peer-reviewed, and Refereed Journals, Impact factor 8.15
Method Development, Validation and Simultaneous Estimation of Ibuprofen and Carisoprodol by RP-HPLC and its Degradation Studies
Authors Name:
Dr D Sri Lakshmi
, G Suji , K Leela Bhaskar , G Sumalatha , P Geetha Bhavani
Unique Id:
IJSDR2101017
Published In:
Volume 6 Issue 1, January-2021
Abstract:
A simple rapid reversed- phase high performance liquid chromatographic method has been developed and validated for estimation of Ibuprofen and Carisoprodol in bulk in pharmaceutical dosage form. Chromatographic separation of Ibuprofen and Carisoprodol was achieved on Waters Alliance -e2695, by using Waters Xterra RP18, (150x4.6mm, 3.5µm) column and the mobile phase containing 2.0 gm Hexane-1-Sulphonic acid is dissolved in 1lt water adjust pH-2.5 with OPA&ACN in the ratio of 50:50% v/v. The flow rate was 1.0 ml/min; detection was carried out by absorption at 240nm using a photodiode array detector at ambient temperature. Retention time for Ibuprofen and Carisoprodol was found to be 2.64 min, 3.13 min respectively. The proposed method was validated according to ICH guidelines. The number of theoretical plates and tailing factor for Ibuprofen and Carisoprodol were NLT 2000 and should not more than 2 respectively. Percentage Relative standard deviation of peak areas of all measurements are always less than 2.0. The method was found to be simple, precise, accurate, robust and suitable for quantitative analysis of Ibuprofen and Carisoprodol and study for its stability.
"Method Development, Validation and Simultaneous Estimation of Ibuprofen and Carisoprodol by RP-HPLC and its Degradation Studies", International Journal of Science & Engineering Development Research (www.ijsdr.org), ISSN:2455-2631, Vol.6, Issue 1, page no.113 - 120, January-2021, Available :http://www.ijsdr.org/papers/IJSDR2101017.pdf
Downloads:
000336257
Publication Details:
Published Paper ID: IJSDR2101017
Registration ID:192841
Published In: Volume 6 Issue 1, January-2021
DOI (Digital Object Identifier):
Page No: 113 - 120
Publisher: IJSDR | www.ijsdr.org
ISSN Number: 2455-2631
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