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INTERNATIONAL JOURNAL OF SCIENTIFIC DEVELOPMENT AND RESEARCH International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2455-2631 | Impact factor: 8.15 | ESTD Year: 2016 open access , Peer-reviewed, and Refereed Journals, Impact factor 8.15 |
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| Paper Title: | Comparison of Standard Tube (ST) method and Congo Red Agar (CRA) method for detection of biofilm production among the isolated species of Coagulase Negative Staphylococci (CONS) in a rural tertiary care hospital. |
| Authors Name: | Dr.Abhishek Debnath , Reshmi Ghosh , Dr. Dipayan Ghosh |
| Unique Id: | IJSDR2008025 |
| Published In: | Volume 5 Issue 8, August-2020 |
| Abstract: | Abstract Introduction: CONS has the ability to colonize the surfaces of biomaterials by adhering in biofilm-structured communities of cells encased in a self-produced polymeric matrix known as biofilm which is responsible for resistance to host defense mechanisms and to the antibiotics. In pathogenesis of infections and drug resistance in CONS infections, biofilms play an important role. Aims and Objectives: To compare Standard Tube (ST) method with Congo Red Agar (CRA) method for detection of biofilm production considering Tissue Culture Method (TCP) as the gold standard method among the isolated species of CONS from clinically significant samples. Materials and Methods: 120 CONS strains isolated from clinically significant samples were identified by different conventional methods and biofilm production was detected by ST and CRA method considering TCP method, the gold standard method. Results: Among 120 CONS isolates, predominant isolated species were S. epidermidis 49(40.83%), S.haemolyticus 30 (25%). 74(61.67%), 64(53.33%) and 40(33.33%) CONS isolates were positive for biofilm production by TCP, ST and CRA method respectively. Comparison of ST method with TCP method by Pearson chi square test showed strong association between TCP and ST and this distribution is statistically significant with P value of 0.006 but CRA method showed poor association between TCP and CRA method and this distribution is statistically significant with P value of <0.01.Conclusion: ST method can be used in routine hospital laboratories for detection of biofilm production as it shows reliable results with good sensitivity and specificity as compared to CRA method |
| Keywords: | Coagulase negative staphylococci (CONS), Congo Red Agar (CRA) method. |
| Cite Article: | "Comparison of Standard Tube (ST) method and Congo Red Agar (CRA) method for detection of biofilm production among the isolated species of Coagulase Negative Staphylococci (CONS) in a rural tertiary care hospital.", International Journal of Science & Engineering Development Research (www.ijsdr.org), ISSN:2455-2631, Vol.5, Issue 8, page no.228 - 233, August-2020, Available :http://www.ijsdr.org/papers/IJSDR2008025.pdf |
| Downloads: | 000337070 |
| Publication Details: | Published Paper ID: IJSDR2008025 Registration ID:192334 Published In: Volume 5 Issue 8, August-2020 DOI (Digital Object Identifier): Page No: 228 - 233 Publisher: IJSDR | www.ijsdr.org ISSN Number: 2455-2631 |
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