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Paper Title: Method Development and Validation For Simultaneous Estimation Of Albuterol and Ipratropium By RP-HPLC Method
Authors Name: Gadi Vijaya Lakshmi , Gangi Sireesha , Mokara Laxmi Priya
Unique Id: IJSDR2006077
Published In: Volume 5 Issue 6, June-2020
Abstract: A simple, Accurate, precise method was developed for the simultaneous estimation of the Albuterol and Ipratropium in Tablet dosage form. Chromatogram was run through Std Altima 150 x 4.6 mm, 5m. Mobile phase containing Buffer0.1%OPA: Acetonitrile taken in the ratio 60:40 was pumped through column at a flow rate of 1 ml/min. Buffer used in this method was 0.1% Perchloric acid buffer. Temperature was maintained at 30°C. Optimized wavelength selected was 270 nm. Retention time of Albuterol and Ipratropium were found to be 2.385 min and 3.218 min. %RSD of the Albuterol and Ipratropium were and found to be 0.5and 0.4 respectively. %Recovery was obtained as 100.11% and 99.71% for Albuterol and Ipratropium respectively. LOD, LOQ values obtained from regression equations of Albuterol and Ipratropium were 0.11, 0.33 and 0.01, 0.03 respectively. Regression equation of Albuterol is y = 6250.x + 1756, y = 12114x + 1191 of Ipratropium. Retention times were decreased and run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.
Keywords: Albuterol, Ipratropium, RP-HPLC
Cite Article: "Method Development and Validation For Simultaneous Estimation Of Albuterol and Ipratropium By RP-HPLC Method ", International Journal of Science & Engineering Development Research (www.ijsdr.org), ISSN:2455-2631, Vol.5, Issue 6, page no.457 - 462, June-2020, Available :http://www.ijsdr.org/papers/IJSDR2006077.pdf
Downloads: 000201506
Publication Details: Published Paper ID: IJSDR2006077
Registration ID:191986
Published In: Volume 5 Issue 6, June-2020
DOI (Digital Object Identifier):
Page No: 457 - 462
Publisher: IJSDR | www.ijsdr.org
ISSN Number: 2455-2631

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