INTERNATIONAL JOURNAL OF SCIENTIFIC DEVELOPMENT AND RESEARCH International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2455-2631 | Impact factor: 8.15 | ESTD Year: 2016
open access , Peer-reviewed, and Refereed Journals, Impact factor 8.15
Objective: To design media from domestic waste with limited nitrogen and high of other nutrients (potassium, phosphorus, vitamins and trace elements) for enhance biomass and bioactive compounds of Chlorococcum humicola with potential application on mosquito control. Methodology: Microalgae Chlorococcum humicola was isolated from lake and cultured using BG11 media. Ingredients were made from domestic waste materials through the processes like collection, shade drying, mincing, cold extraction and sterilization by membrane filtration method. The items were BG11 media, BG11 with ingredients of designed media, and designed media with optimum concentration of ingredients inoculated with 5% of inoculum. Growth was monitored from 0th day to 25th day by the measurement of optical density, chlorophyll content and dry weight. Larvicidal evaluation were done by exposing 4-6 larva into 100ppm, 200ppm, 300ppm, 400ppm, 500 ppm concentrations of ethanolic algal extract of Chlorococcum humicola. Result and discussion: Chlorococcum humicoal was isolated from lake water and identified, the diverse parameters of growth was measured in three different media during the 0 to 25 days, gradual increase were showed in algae cells(optical density), chlorophyll content and final weight of algal biomass. Complete mortality of larva species were seen in 500ppm bestowed the efficacy of Chlorococcum humicola to kill mosquito species with small concentrations which was cultivated in the designed media compared to BG11 and BG11 with ingredients of designed media. From this study, with limited nitrogen source and optimum level of nutrients such as potassium, phosphorus, vitamins, trace elements from different domestic waste materials was highly supported the growth of Chlorococcum humicola and improved their efficacy for potential applications. Key words: Chlorococcum humicola, nutrient, media development, applications, larvicidal, Mosquito control, Domestic.
Keywords:
Chlorococcum humicola, nutrient, media development, applications, larvicidal, Mosquito control, Domestic.
Cite Article:
"Designing Media from Domestic Waste to Enhance Growth of Chlorococcum humicola and Its Application on Mosquito Control", International Journal of Science & Engineering Development Research (www.ijsdr.org), ISSN:2455-2631, Vol.5, Issue 5, page no.102 - 111, May-2020, Available :http://www.ijsdr.org/papers/IJSDR2005020.pdf
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000337071
Publication Details:
Published Paper ID: IJSDR2005020
Registration ID:191716
Published In: Volume 5 Issue 5, May-2020
DOI (Digital Object Identifier):
Page No: 102 - 111
Publisher: IJSDR | www.ijsdr.org
ISSN Number: 2455-2631
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