Study the Effect of Indian Gentian, Genoprotective Agent in Type 2 Diabetes Mellitus Patients

Priyanka Sharma , Dr. Ravinder Pal Singh

Indian Gentian, T2DM, Comet Assay, SCEs, Protein Oxidation,DNA etc

The present study was undertaken to study the effect of Indian Gentian, a herb, as a genoprotective agent in type 2 diabetes mellitus (T2DM) patients. For this, a total of 52 T2DM patients were investigated, of which 38 received 500 mg - 1 g Indian Gentian thrice daily escalated over three months (Group 1). The remaining 14 patients were not given the herb (Group 2). Fifteen age and sex matched non diabetic healthy volunteers served as controls (Group 3). All three groups were studied for DNA damage by comet assay and Sister Chromatid Exchanges (SCEs); Group 1 was also investigated for protein oxidation. Paired and unpaired t tests were performed at 95% confidence interval. Results of comet assay and SCE studies revealed that in Group 1, post Indian Gentian treatment, normal cell population increased, whereas moderately damaged, highly damaged and apoptotic cell population and SCE decreased as compared to Group 1 (pre-treatment patients) and Group 2 (without treatment patients). In comet assay, statistically significant difference between Group 1 (post-treatment patients) and Group 3 (controls) suggested that the herb was able to decrease the DNA damage but not as low as non-diabetic healthy controls. On the other hand, SCE analysis showed that the herb can reduce such exchanges to as low as the controls. In protein oxidation assay, no significant difference was found between the pre- and post-treatment T2DM patients of Group 1. The present study therefore indicated that overall Indian Gentian may have a significant effect on reducing DNA damage and attenuating SCEs in T2DM patients.

Published Paper   E-Certificate

"Study the Effect of Indian Gentian, Genoprotective Agent in Type 2 Diabetes Mellitus Patients", IJSDR - International Journal of Scientific Development and Research (www.IJSDR.org), ISSN:2455-2631, Vol.4, Issue 6, page no.53 - 58, June-2019, Available :https://ijsdr.org/papers/IJSDR1906013.pdf

Volume 4 Issue 6, June-2019

Pages : 53 - 58

Paper Reg. ID: IJSDR_190640

Published Paper Id: IJSDR1906013

Downloads: 000347369

Research Area: Biological Science

Country: Mahaendragarh, haryana, India

Published Paper PDF: https://ijsdr.org/papers/IJSDR1906013

Published Paper URL: https://ijsdr.org/viewpaperforall?paper=IJSDR1906013

ISSN: 2455-2631 | IMPACT FACTOR: 9.15 Calculated By Google Scholar | ESTD YEAR: 2016

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 9.15 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Publisher: IJSDR(IJ Publication) Janvi Wave

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