IJSDR
IJSDR
INTERNATIONAL JOURNAL OF SCIENTIFIC DEVELOPMENT AND RESEARCH
International Peer Reviewed & Refereed Journals, Open Access Journal
ISSN Approved Journal No: 2455-2631 | Impact factor: 8.15 | ESTD Year: 2016
open access , Peer-reviewed, and Refereed Journals, Impact factor 8.15

Issue: April 2024

Volume 9 | Issue 4

Impact factor: 8.15

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Paper Title: Biophysical study of Brucella abortus on electrochemical platform
Authors Name: Ajay Kumar Gupta , Vepa Kameswara Rao , Duraipandian Thava Selvam
Unique Id: IJSDR1704010
Published In: Volume 2 Issue 4, April-2017
Abstract: Study of biophysical interactions have been carried out using specific combination of proteins such as Brucella abortus cell envelop protein (as antigen) and its complementary antibody (raised in mice and rabbit). A best optimized composition of biocompatible CeO2 nano-octahedra and other biocompatible materials (chitosan) was used in this study. Linear range was found in the range of 6.25 µg/mL–100 µg/mL. Detection limit was found 0.1 ng/mL. By conventional plate ELISA it was possible to detect 50 µg/mL only. Experiments were conducted using 110 suspected patient serum samples and results were compared with ELISA and Rose Bengal Plate test (RBPT). We also studied the shelf life of prepared immunosensor. The shelf life of immunosensor was found to be 51 days.
Keywords: Biophysics; Electrochemistry, ELISA; CeO2 nano-octahedra; CE-protein antigen
Cite Article: "Biophysical study of Brucella abortus on electrochemical platform", International Journal of Science & Engineering Development Research (www.ijsdr.org), ISSN:2455-2631, Vol.2, Issue 4, page no.54 - 67, April-2017, Available :http://www.ijsdr.org/papers/IJSDR1704010.pdf
Downloads: 000337067
Publication Details: Published Paper ID: IJSDR1704010
Registration ID:170160
Published In: Volume 2 Issue 4, April-2017
DOI (Digital Object Identifier): http://www.dx.doi.org/10.6084/m9.figshare.4833437
Page No: 54 - 67
Publisher: IJSDR | www.ijsdr.org
ISSN Number: 2455-2631

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