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INTERNATIONAL JOURNAL OF SCIENTIFIC DEVELOPMENT AND RESEARCH International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2455-2631 | Impact factor: 8.15 | ESTD Year: 2016 open access , Peer-reviewed, and Refereed Journals, Impact factor 8.15 |
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| Paper Title: | Detection of cnf1 gene for the presence of Cytotoxic Necrotising Factor among clinical isolates of Escherichia coli |
| Authors Name: | Shabana Bowsiya , Dr. Naveen Kumar |
| Unique Id: | IJSDR2002014 |
| Published In: | Volume 5 Issue 2, February-2020 |
| Abstract: | Escherichia coli (E.coli) is a gram-negative, facultative aneaerobic rod-shaped bacteria that is a commensal of the human gut. They are beneficial to the host by producing Vitamin K (k6) and prevent pathogenic bacteria from colonizing. Uropathogenic strains of E.coli is also a commensal of the gut. Toxigenic strains of E. coli are of three types such as Enterotoxigenic E. coli (ETEC), Shiga toxigenic E. coli (STEC) and necrotoxigenic E. coli (NTEC). Two different types of NTEC have been reported they are NTEC1 and NTEC2 depending on the toxin they produce [1] Cytotoxic necrotizing factor 1 (CNF1) is produced by NTEC1 and cytotoxic necrotizing factor 2 (CNF2) is produced by NTEC2. CNF1 is chromosomally encoded, whereas CNF2 is coded by genes located on the Vir plasmid. A sum of 20 urinary isolates of E. coli were subjected to antibiotic susceptibility testing and detected for cnf1 gene by PCR. We found increased percentage of isolates were shown to be resistant to different antibiotics and 70% of them were resistant to imipenem drug. 60% of them were harboring cnf1 gene by PCR. This indicates that most of the strains of Uropathogenic E. coli were found to harbor cnf1 gene, is associated with the implication of UTI in humans. Certain strains of E.coli are known to produce amyloid material which are similar to those seen in Alzhiemer’s disease [14]. The versatility and the ease of handling this organism makes it easier to do extensive studies. Futher studies need to be conducted to gain better understanding about its ecology and natural history. |
| Keywords: | E. coli, cnf1 gene, PCR, cytotoxic necrotizing factor, Alzhiemer’s disease |
| Cite Article: | "Detection of cnf1 gene for the presence of Cytotoxic Necrotising Factor among clinical isolates of Escherichia coli", International Journal of Science & Engineering Development Research (www.ijsdr.org), ISSN:2455-2631, Vol.5, Issue 2, page no.73 - 76, February-2020, Available :http://www.ijsdr.org/papers/IJSDR2002014.pdf |
| Downloads: | 000337069 |
| Publication Details: | Published Paper ID: IJSDR2002014 Registration ID:191269 Published In: Volume 5 Issue 2, February-2020 DOI (Digital Object Identifier): Page No: 73 - 76 Publisher: IJSDR | www.ijsdr.org ISSN Number: 2455-2631 |
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