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INTERNATIONAL JOURNAL OF SCIENTIFIC DEVELOPMENT AND RESEARCH International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2455-2631 | Impact factor: 8.15 | ESTD Year: 2016 open access , Peer-reviewed, and Refereed Journals, Impact factor 8.15 |
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| Paper Title: | Molecular Characterization of Novel Symbiotic bacteria from Entomopathogenic nematodes |
| Authors Name: | Deepa I , Mohandas C , Siji JV |
| Unique Id: | IJSDR1908007 |
| Published In: | Volume 4 Issue 8, August-2019 |
| Abstract: | Novel entomopathogenic nematodes (EPN) belonging to the genus Rhabditis (Family Rhabditidae) were isolated from different parts of the country for the first time. Each isolate is associated with a specific bacterium. About 65 isolates of such nematodes are maintained alive in CTCRI. Bacteria isolated from these are different from one another based on morphology and biochemical tests. In the present study we have isolated twelve different symbiotic bacteria from surface sterilized infective juveniles of entomopathogenic nematode isolates collected from different parts of India. Sequencing of 16S rDNA of 12 isolates indicated that all are different from one another and belong to the following seven genera viz. Acinetobacter, Bacillus, Comamonas, Stenotrophomonas, Achromobacter, Klebsiella and Brucellaceae. To analyze the genetic variability among the bacterial isolate DNA fingerprinting technique like ARDRA (Amplified Ribosomal DNA Restriction Analysis) was used. Nucleotide sequences of nine isolates have been submitted to NCBI database and the following accession numbers were allotted: HQ200405, HQ200406, HQ200408, HQ200409, HQ200410, HQ200411, HQ200412, HQ200414 and HQ200415. |
| Keywords: | Entomopathogenic nematodes (EPN), Rhabditis, E.coli, phylogenetic tree, ARDRA (Amplified Ribosomal DNA Restriction Analysis). |
| Cite Article: | "Molecular Characterization of Novel Symbiotic bacteria from Entomopathogenic nematodes", International Journal of Science & Engineering Development Research (www.ijsdr.org), ISSN:2455-2631, Vol.4, Issue 8, page no.38 - 43, August-2019, Available :http://www.ijsdr.org/papers/IJSDR1908007.pdf |
| Downloads: | 000337068 |
| Publication Details: | Published Paper ID: IJSDR1908007 Registration ID:190866 Published In: Volume 4 Issue 8, August-2019 DOI (Digital Object Identifier): Page No: 38 - 43 Publisher: IJSDR | www.ijsdr.org ISSN Number: 2455-2631 |
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