INTERNATIONAL JOURNAL OF SCIENTIFIC DEVELOPMENT AND RESEARCH International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2455-2631 | Impact factor: 8.15 | ESTD Year: 2016
open access , Peer-reviewed, and Refereed Journals, Impact factor 8.15
A new stability-indicating high performance liquid chromatographic method was developed and validated for the determination of Hydrocortisone and Ketoconazole in a tablet dosageform. The separation was achieved on a reverse phase C18 column (Agilent ODS) (250mm × 4.6 mm, 5 µ m) with mobile phase consisted of methanol:water (61:39 % v/v containing 0.2% HSA, pH 3.0 adjusted with Orthophosphoric acid) and the eluents were detected at 221nm. The retention time of Hydrocortisone and Ketoconazole was 6.0 min and 26.2 min respectively with the flow rate of 1mL.min-1. Drugs were subjected to stress conditions of acidic, basic, oxidation, photolytic, neutral and thermal degradation and considerable degradants were detected in all stress conditions. A total of 17 degradation products were observed and well separated from analyte peaks, hence the method could effectively employed as stability-indicating one. The response of linear was in the range 5-50 µg/mL for Hydrocortisone and 10 to 70 µg/mL for Ketoconazole, respectively. The relative standard deviation values for intra- and interday precision studies were 1.06 % and 1.5 % for Hydrocortisone and 0.46 % and 1.1% for Ketoconazole.Recoveries ranged in between 98-102% for both Hydrocortisone and Ketoconazole..
"Stability Indicating RP- HPLC - DAD Method for the Simultaneous Estimation of Hydrocortisone and Ketoconazole in Tablet Dosage form.", International Journal of Science & Engineering Development Research (www.ijsdr.org), ISSN:2455-2631, Vol.4, Issue 1, page no.218 - 228, January-2019, Available :http://www.ijsdr.org/papers/IJSDR1901038.pdf
Downloads:
000337348
Publication Details:
Published Paper ID: IJSDR1901038
Registration ID:190034
Published In: Volume 4 Issue 1, January-2019
DOI (Digital Object Identifier):
Page No: 218 - 228
Publisher: IJSDR | www.ijsdr.org
ISSN Number: 2455-2631
Facebook Twitter Instagram LinkedIn