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INTERNATIONAL JOURNAL OF SCIENTIFIC DEVELOPMENT AND RESEARCH International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2455-2631 | Impact factor: 8.15 | ESTD Year: 2016 open access , Peer-reviewed, and Refereed Journals, Impact factor 8.15 |
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| Paper Title: | Electrochemical study of biophysical interaction of Brucella abortus antigen |
| Authors Name: | Ajay Kumar Gupta , Vepa Kameswara Rao , Duraipandian Thava Selvam |
| Unique Id: | IJSDR1704009 |
| Published In: | Volume 2 Issue 4, April-2017 |
| Abstract: | Study of biophysical interactions have been carried out using specific combination of proteins such as Brucella abortus cell envelop protein (as antigen) and its complementary antibody (raised in mice and rabbit). MWCNT Paste electrodes (MWCNTPEs) were used in this study. Linear range was found in the range of 2.5 µg/mL–100 µg/mL. The detection limit was found 1 ng/mL. By ELISA it is possible to detect 50 µg/mL only. Experiments were conducted using 110 suspected patient serum samples and results were compared with ELISA and Rose Bengal Plate test (RBPT). The immunosensor was found to be highly sensitive compared to ELISA and RBPT. We also studied the shelf life of prepared immunosensor. The shelf life of immunosensor was found to be 44 days. |
| Keywords: | Immunosensor; ELISA; MWCNT-paste electrode; CE-protein antigen |
| Cite Article: | "Electrochemical study of biophysical interaction of Brucella abortus antigen", International Journal of Science & Engineering Development Research (www.ijsdr.org), ISSN:2455-2631, Vol.2, Issue 4, page no.43 - 53, April-2017, Available :http://www.ijsdr.org/papers/IJSDR1704009.pdf |
| Downloads: | 000337211 |
| Publication Details: | Published Paper ID: IJSDR1704009 Registration ID:170159 Published In: Volume 2 Issue 4, April-2017 DOI (Digital Object Identifier): http://www.dx.doi.org/10.6084/m9.figshare.4833440 Page No: 43 - 53 Publisher: IJSDR | www.ijsdr.org ISSN Number: 2455-2631 |
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